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Figure 1. A) A gold particle (arrow) labeling S6 is entering the nuclear pore. The nuclear envelope in yellow (B) follows the line of ribosomes on the outer membrane. The protein is not associated with RNA (absence of terbium staining) and hence is not part of the SSU. S6 protein molecule then reaches IG clusters (C), moves to CBs (D, CB), and from there to the nucleolus (E, N), first on the DFC. In the nucleolar granular component (F), S6 has already colocalized with myosin (large grains). The SSU moves in the nucleoplasm (G, H: the terbium-stained RNA is underlined in magenta). S6 (small grains), associated with RNA, colocalizes with NMI (large grain, I, J). K) Large (NMI) and small grains (actin) surround the intermediate sized-grains corresponding to S6. The arrow indicates an electron-dense particle that could correspond to a single SSU. SSU reaches the nuclear pores (L, arrows) associated with RNA (M, N) and accompanied by NMI (O, P). Bar represents 100 nm (A–F and L–N); 50 nm (G–J, O, P); 25 nm (K).
