Table 1. Transposition assay data from the use of chimeric transposases and modified target plasmids.
| Experiment |
Total Donor plasmids recovereda |
Total number of transposition events |
Transposition frequencyb |
Fold Increasec |
|
| Control Mos helper + pGDV1 |
4.59 x 106 |
68 |
1.48 x 10-5 |
— |
| Gal4-Mos helper + pGDV1 |
2.08 x 106 |
48 |
2.32 x 10-5 |
1.6 |
| Gal4-Mos helper + pGDV1-UAS |
1.80 x 105 |
53 |
2.96 x 10-4 |
20 (12.7) |
| Gal4-piggyBac helper + pGDV1 |
6.12 x 105 |
12 |
1.96 x 10-5 |
— |
| Gal4-piggyBac helper + pGDV1-UAS |
2.93 x 105 |
67 |
2.28 x 10-4 |
11.6 |
|
a The total number of donor plasmids recovered is estimated by the number of Amp resistant colonies recovered.
b The transposition frequency is calculated by dividing the number of confirmed transposition products recovered, by the total number of donor plasmids recovered. These data are the cumulative data from 3 independent injection and recovery experiments.
c The fold increases for the Mos1 experiments are relative to the Control Moshelper + pGDV1 experiment except for the number in parentheses, which represents the fold increase in transposition when using pGDV1-UAS target plasmid, compared to the standard pGDV1 target plasmid. The fold increase for the piggyBac experiment is based on the increase observed when using the pGDV1-UAS target plasmid compared to the standard pGDV1 target plasmid.
