Click on image to view larger version.
Figure 1. Schematic map of the pGDV1-UAS target plasmid showing integrations of Mos1 donor elements when using the Gal4-Mos1 chimeric transposase. Open circles represent insertions of the Mos1 donor element into the standard pGDV1 target plasmid, which lacks the UAS target. The solid circles represent insertions in the opposite orientation with respect to the Chloramphenicol (Cam) resistance gene. 48 insertions were recovered from these control experiments. The "cloverleaf" (
) and solid arrow represent the predominant site hit by Mos1 donor elements when using the pGDV1-UAS target plasmid. As indicated above the solid arrow, this site was hit 51 times during these assays. Two additional insertions recovered when using the pGDV1-UAS target plasmid are indicated by up and down arrowheads. Both experiments shown here utilized the chimeric Gal4-Mos1 transposase helper plasmid. For clarity, the 68 insertions recovered from the assays using the unmodified Mos helper plasmid are not shown; however, they show a distribution similar to the open and solid circles. The numbers represent the nucleotide location on the pGDV1 plasmid. The UAS target site is shown as a shaded box, the Chloramphenicol (Cam) resistance gene as an open box. Insertions into the antibiotic resistance gene would not be recovered from this assay due to the selection regime used to remove false positives and recombination events.
