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Figure 1. A) Evidence for integrin 
Vß3 as a binding site for resveratrol. MCF-7 cells were treated for 4 h with resveratrol (RV, 10 µM) in the presence or absence of RGD peptide (5–500 nM) or RGE peptide (500 nM). Nucleoproteins of treated cell samples were separated by gel electrophoresis and immunoblotted with antibodies to serine-15-phosphorylated 53 (pSer15-p53) or phosphorylated ERK1/2 (pERK1/2). Immunoblots shown in the figures are representative of three experiments, and the upper graph shows the mean ± SEM of change from control image intensities normalized to a value of 1. A lamin (Lamin B) immunoblot provides a loading control. MCF-7 cells treated for 24 h in the same manner were studied by nucleosome ELISA as a measure of apoptosis. The lower graph summarizes the results of three experiments. B) Effect of Ab to integrin Vß3 on RV action. MCF-7 cells were treated with either anti-Vß3 (1–20 µg/ml) or anti-Vß5 (20 µg/ml) for 24 h with or without RV (10 µM) added for the last 4 h. Anti-Vß3 and anti-Vß5, each alone, was used as a negative control. C) MDA-MB231 cells were treated with either anti-Vß3 (10–3–0.1 µg/ml) or anti-Vß5 (0.1 µg/ml) for 24 h with or without RV (10 µM) for the last 4 h. Anti-Vß3 and anti-Vß5, in the absence of RV, were used as negative controls. Results are presented as in B).
