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Figure 2. Effect of MMP-1 on Tat-induced LTR transactivation. SVGA-long terminal repeat-GFP cell cultures were exposed to Tat and MMP-1, either alone or in combination, with or without FN-439. Transactivation of LTR was measured 24 h later. Tat alone caused significant transactivation compared to untreated controls (P<0.05). When Tat was incubated with MMP-1, transactivation returned to that seen with no treatment (P<0.05). When Tat, MMP-1, and FN-439 were all incubated together, transactivation reappeared (P<0.05). MMP-1 alone and FN-439 alone had no significant effect on transactivation. Concentrations: Tat (200 nM), MMP-1 (40 ng/µl), FN-439 (0.5 µg/µl). Data represent mean ± SE.
