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Figure 2. Intramuscular injection of biglycan core polypeptide into bgn null skeletal muscle restores sarcolemmal expression of 
-dystrobrevin-1 and -2 and ß1- and ß2-syntrophin. Exogenous biglycan core polypeptide increases the sarcolemmal expression of -dystrobrevin-1 and -2 and ß1- and ß2-syntrophin in vivo. Two-wk-old bgn null mice were injectedintramuscularly into the right quadriceps femoris muscles with 50 µg biglycan core and into the left quadriceps with buffer alone. 1.0% india ink was added to each injected solution to allow visualization of the injection site. Muscle was harvested 11 days after injection, sectioned, and immunolabeled. The injections were performed using three bgn null litters (14 animals total). Biglycan core polypeptide enhances sarcolemmal expression of -dystrobrevin-1 (-DB-1) (A), -dystrobrevin-2 (-DB-2) (B), ß1-syntrophin (ß1-Syn) (C), and ß2-syntrophin (ß2-Syn) (D). The injected biglycan core polypeptide colocalizes with -dystrobrevin and ß-syntrophin expression at the sarcolemma of injected muscle. Scale bar = 20 µM.
