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Figure 1. CTGF stimulates phosphorylation of p27Kip-1 on serine 10 in an Akt/PKB-dependent manner. We visualized the subcellular localization of p27Kip-1 in response to CTGF by immunocytochemistry. Serine 10 phosphorylated p27Kip-1 was primarily localized in the nucleus in untreated cells. Stimulation with CTGF led to a clear cytoplasmic accumulation of serine 10-phosphorylated p27Kip-1 in a Akt/PKB-sensitive manner; addition of the Akt/PKB inhibitor IL-6-hydroxymethyl-chiro-inositol-2(R)-2-methyl-3-O-octadecylcarbonate resulted in nuclear localization (A). Cytoplasmic serine 10-phosphorylated p27Kip-1 appeared to colocalize with the ends of shortened actin fibers in CTGF treated cells (B). CTGF also stimulated the Akt/PKB dependent phosphorylation of the p27Kip-1 transcriptional regulator FKHR (C).
