Click on image to view larger version.
Figure 2. Gene expression in brain pericytes. In situ hybridization on sections from E14.5 wild-type embryos using probes for RGS5 (A, B) Kir6.1 (C, D), DLK (E, F), and SUR2 (G, H). The in situ mRNA signal (blue) is shown on sections from the brain in which the endothelial cells are labeled using isolectin B4 (brown). Note that the cells positive for any of the four mRNAs are closely apposed to, but distinct from, the endothelium (arrows). Most of the endothelial abluminal surface is free from coverage by the mRNA positive cells (arrowheads). Thus, the distribution and density of the cells positive for RGS5, Kir6.1, DLK, and SUR2 is compatible with expression in pericytes.
