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Figure 2. Ionomycin stimulation of GlcAT-I promoter is dependent on the activation of the MAPK pathway. A) Cells were treated with 1 µM ionomycin for 0–120 min and phosphorylation of ERK was monitored by immunoblot using antiphospho-ERK1/2 antibodies. Equal expression of ERK1/2 in the different samples was confirmed using anti-ERK1/2 antibodies. B) Cells were pretreated or not with PD98059 (30 µM) 30 min before ionomycin exposure. Five minutes later, cell extracts were analyzed by immunoblotting using antiphospho-ERK1/2 and anti-ERK1/2 antibodies. C) Cells were transfected with pGL-302 construct, pretreated or not with PD98059 (30 µM) 30 min, then treated or not with 1 µM ionomycin for 4 h. Cell extracts were assayed for luciferase activity.
