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Figure 2. SOD1 down-regulation affects mitochondrial homeostasis. A) SH-SY5Y cells were transfected with siSOD or with siScr. After 48 h, cells were stained with 200 nM TMRE and used for cytofluorimetric analyses as described in Materials and Methods. Histograms represent 6 with similar results. B) After 48 h from transfection, siSOD and siScr cells were used for ATP measurement by a bioluminescence detection kit as described in Materials and Methods. Data are expressed as nmol/mg prot (bars, mean±SD; n=10; *P=0.002). C) After 24 h from transfection, siSOD and siScr cells were treated with 0.5 µM rotenone for the next 24 h. Viable cells number was determined by trypan blue exclusion (bars, mean±SD; n=5; **P<0.001)
