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,1
* Department of Physiology, Queen’s University, Kingston, Ontario K7L 3N6, Canada; and
Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, Massachusetts USA
1Correspondence: Department of Physiology, Queen’s University, Kingston, Ontario K7L 3N6, Canada. E-mail: melol{at}post.queensu.ca
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONStrategies for genetic...Targets for gene therapy...Gene therapy for myocardial...Gene therapy for myocardial...Gene therapy for rescue...Gene therapy for inherited...Cell-based therapy for...Tissue engineeringPerspectives and future...REFERENCES |
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Key Words: coronary artery disease • AAV • myocardial protection • contractile function
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONStrategies for genetic...Targets for gene therapy...Gene therapy for myocardial...Gene therapy for myocardial...Gene therapy for rescue...Gene therapy for inherited...Cell-based therapy for...Tissue engineeringPerspectives and future...REFERENCES |
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, 2)
. The complexity of the pathological processes leading to CAD and the lack of specific predictive markers have been major impediments to the development of effective preventive therapies despite the identification of various risk factors and sensitive risk assessment technologies (3
, 4)
. Consequently, the focus has been on the design of "rescue" treatments for overt symptoms of the disease such as myocardial ischemia, left ventricular pump failure, and hemodynamic overload (5
, 6)
. Ironically, the improved survival of MI patients treated with these therapies has led directly to a dramatic increase in the number of patients suffering from heart failure (1
, 2
, 7)
, indicating the need for more effective therapies.
The availability of cardiotropic vector systems such as adeno-associated virus (AAV) capable of sustained expression of therapeutic proteins (8)
, together with the identification of new targets for therapeutic intervention (9)
and the recent isolation of bone marrow-derived progenitor cells with regenerative potential (10)
, offers opportunities for the design of gene therapies for myocardial protection and rescue. Delivery of antioxidant, proangiogenic and contractility-enhancing genes may have potential as therapy for patients afflicted or at risk of developing MI and HF (11
12
13)
, whereas transplantation of autologous progenitor cells may have therapeutic value in revascularization and repair of ischemic and infarcted myocardium (14)
. Evaluation of these experimental gene and cell-based strategies for myocardial protection, rescue, and regeneration are being intensely pursued by various groups, and several small-scale trials support the feasibility of these approaches. In this article we review the major advances in gene and cell-based therapies for heart disease, with emphasis on strategies for protection and rescue of the ischemic and failing myocardium, their clinical feasibility, and a perspective on future developments in the field.
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Strategies for genetic manipulation of the myocardium |
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TOPABSTRACTINTRODUCTIONStrategies for genetic...Targets for gene therapy...Gene therapy for myocardial...Gene therapy for myocardial...Gene therapy for rescue...Gene therapy for inherited...Cell-based therapy for...Tissue engineeringPerspectives and future...REFERENCES |
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) (15
16
17
18
19)
. The most common somatic gene therapy strategy for the myocardium involves the exogenous delivery and expression of genes whose endogenous activity may be either defective or attenuated due to a mutation or a pathological process, in order to restore the function of the deficient or undercompensating gene. In this setting, a full-length or partial cDNA encoding the deficient gene is delivered to the target tissues using a vector system capable of expressing the therapeutic protein. Such gain-of-function gene transfer strategies have been widely used with a variety of therapeutic genes, including proangiogenic and survival factors (20
, 21)
, antioxidant enzymes (12)
and anti-inflammatory cytokines (22)
. In other instances, the silencing of genes involved in the pathological processes may be desirable. Acute blockade of gene transcription can be achieved by treatment with short single-stranded antisense oligodeoxynucleotides, ribozymes, and, more recently, using RNA interference technology. (16
, 17
, 23
24
25)
. These molecules inhibit the synthesis of proteins by hybridizing in a sequence-specific complementary fashion to target mRNA. Inhibition of transcription factor DNA binding using double-stranded decoy oligonucleotides containing DNA consensus binding sequences for target transcriptional factors has also been used (for a review, see ref 16
). The decoy is usually delivered in molar excess, sequestering the target transcription factor and rendering it incapable of binding to the promoter region of the target gene. In many instances, short-term inhibition of a pathogenic gene is sufficient to prevent the development or progression of disease. For example, the inhibition of cell cycle regulatory proteins using decoy oligonucleotides was shown to prevent neointimal hyperplasia and subsequent restenosis after balloon angioplasty or bypass grafting (16)
. Recently, nucleic acid and peptide aptamers have been developed that are capable of inhibiting protein function without altering the genetic complement of the host (26)
. However, the use of aptamers in cardiovascular therapeutics has not been evaluated.
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The choice of therapeutic target, vector and delivery strategy is governed to a large extent by the pathological features of the disease, the putative role of the target gene(s) in the pathophysiological process, and the timing of intervention (15)
. The efficiency of gene transfer to the myocardium is highly dependent on the type of vector, route, and the dosage and volume of delivery of the genetic material. (27
, 28)
. A number of vector systems have evolved over the years (Table 1
). Nonviral vectors, which include naked plasmids, cationic liposome formulations, synthetic peptides, and several physical methods, usually yield low and transient gene transfer efficiency due to lack of genomic integration and rapid degradation of the vector (19)
. A promising new delivery strategy uses synthetic peptide carriers containing a nuclear localization signal to facilitate nuclear uptake of the target cDNA (29)
. These peptide–DNA heteroplexes are recognized by intracellular receptor proteins and imported into the nucleus, where the target cDNA is transcribed.
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Recombinant viruses have become the preferred vectors for myocardial gene transfer (Table 1)
. These are replication-deficient viral particles that retain their ability to penetrate target cells and deliver genetic material with much higher efficiency than nonviral vectors (18
, 30)
. Some vectors, such as AAV and lentivirus, are capable of sustained expression of the therapeutic gene (8
, 18
, 30)
, which may be essential for the design of therapies for chronic myocardial disease. Unfortunately, a robust immune reaction may be triggered by the host in response to the viral proteins synthesized by viral vectors that may reduce the efficiency of gene transfer and the sustainability of transgene expression (18)
. There is a risk, albeit remote, that these vectors may revert to replication proficiency, thus raising safety concerns about biological hazards such as oncogenesis and insertional mutagenesis (18
, 30)
. The overall safety and specificity of gene transfer protocols could, however, be enhanced by incorporating regulatory elements capable of directing tissue-specific expression as well as regulated expression of the transgene in response to underlying pathophysiological cues such as hypoxia, oxidative stress, or inflammation (31)
.
Several routes have been used to administrate the therapeutic material to the myocardium (Fig. 2
). Intracoronary delivery of the therapeutic gene is the preferred route for global myocardial diseases such as heart failure and cardiomyopathy. However, the selectivity of coronary endothelium and the barrier imposed by the basement membrane may restrict the diffusion of some vectors and limit distribution and uptake of the therapeutic transgene. In contrast, intramyocardial injection may be a desirable method for gene delivery to areas of regional myocardial disease. This approach has been used to deliver angiogenic and cytoprotective genes to ischemic myocardium (12
, 15)
. However, transgene expression is restricted to the area surrounding the site of injection, requiring in some cases multiple injections to adequately cover the affected area. A variety of catheter types have been developed for both intracoronary and intramyocardial delivery with the assistance of trans-esophageal echocardiographic guiding and mapping techniques (15
, 32)
. Other methods, such as pericardial injection and retroperfusion, have had limited application in myocardial gene transfer (33)
.
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Targets for gene therapy for myocardial protection and rescue |
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TOPABSTRACTINTRODUCTIONStrategies for genetic...Targets for gene therapy...Gene therapy for myocardial...Gene therapy for myocardial...Gene therapy for rescue...Gene therapy for inherited...Cell-based therapy for...Tissue engineeringPerspectives and future...REFERENCES |
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). In the setting of myocardial protection, the overexpression of cytoprotective and survival genes such as antioxidant enzymes (11
, 34
, 35)
, antiapoptotic proteins (36)
, protein kinase B/Akt (21)
, and/or the inhibition of proinflammatory cytokines (22)
, proapoptotic (37)
, and prooxidant (38)
genes have emerged as potential therapeutic targets for cardioprotection from studies in various animal and cellular models of myocardial ischemic injury.
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Gene therapy strategies for rescuing failing myocardium may be attainable in certain conditions (Table 2)
. Therapeutic angiogenesis by delivery of genes coding for proangiogenic growth factors has been shown to promote neovascularization and functional recovery of ischemic myocardium in several animal models and in humans with coronary artery disease (12
, 15
, 20
, 39
40
41)
. Strategies in the postinfarction period may include inhibition of genes involved in regulation of ventricular remodeling and chamber dilatation such as the matrix metalloproteinases (MMPs) that participate in extracellular matrix degradation (42)
. Potential strategies for rescuing contractile function in the failing myocardium include overexpression of the sarcoplasmic reticulum calcium ATPase (SERCA2a) (43)
, ß-adrenergic receptor (44)
, and adenylate cyclase (45)
(Table 2)
. An exciting new field is emerging with the recent identification and isolation of endothelial and cardiomyocyte precursor stem cells from adult bone marrow (46)
; the ability to expand and genetically modify these cells ex vivo offers the opportunity to use them as an autologous cellular substrate for repair of infarcted myocardium.
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Gene therapy for myocardial protection |
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TOPABSTRACTINTRODUCTIONStrategies for genetic...Targets for gene therapy...Gene therapy for myocardial...Gene therapy for myocardial...Gene therapy for rescue...Gene therapy for inherited...Cell-based therapy for...Tissue engineeringPerspectives and future...REFERENCES |
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(Fig. 3
). Reoxygenation of the ischemic myocardium increases the formation of reactive oxygen species (ROS) formation, which may eventually deplete the buffering capacity of endogenous antioxidant systems (48)
. The development of gene therapies for acute myocardial infarction has been difficult because the time required for transcription and translation of therapeutic genes with the current generation of vectors exceeds the window for successful intervention. For this reason, gene transfer of anticoagulant genes is not feasible as primary thrombolytic therapy for acute myocardial infarction. An alternative gene therapy approach for myocardial protection is to devise a strategy that could prevent I/R injury by using a method that could confer long-term expression of cytoprotective genes in the myocardium (Table 2)
. This novel concept of preventive gene therapy would protect the heart from future I/R injury, thereby minimizing the need for acute intervention (11)
.
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Gene therapy aimed at increasing endogenous antioxidant reserves should, in principle, be a useful strategy given the prominent role of oxidative stress in CAD and I/R injury (47)
. Such a strategy may potentiate the native protective response of the myocardium, rendering it resistant to future ischemic insults. We have evaluated the feasibility of antioxidant enzyme gene transfer as a long-term first line of defense against I/R-induced oxidative injury using an rAAV vector for intramyocardial delivery of heme oxygenase-1 (HO-1), an enzyme that is involved in the catabolism of heme (12)
. Our findings show that HO-1 gene delivery to the left ventricular risk area several weeks in advance of myocardial infarction in a rat model of myocardial I/R injury results in 
80% reduction in infarct size (Fig. 4
). The reduction in myocardial injury in the treated animals is accompanied by decreases in oxidative stress, inflammation, and interstitial fibrosis, leading to postinfarction functional recovery and normalization of left ventricular dimensions (Fig. 4)
. Comparable findings were seen with extracellular superoxide dismutase (ecSOD) gene transfer (36
, 49)
. This secreted metalloenzyme plays an essential role in maintenance of redox homeostasis by dismutating the oxygen free radical superoxide. Our findings showed improved long-term survival after MI in the ecSOD-treated animals relative to the animals treated with the control vector in parallel with smaller infarcts and decreased myocardial inflammation (49)
. Significant protection from I/R injury has also been achieved by overexpression of other major antioxidant enzyme systems such as Cu/Zn SOD (50)
, catalase (51)
, and glutathione peroxidase (52)
, stress-induced heat shock proteins (53)
, survival genes (Bcl-2, Akt) (21
, 54)
, as well as immunosuppressive cytokines (22)
, adenosine A1 and A3 receptors (55)
, kallikrein (56)
, caspase inhibitor (37)
, and hepatocyte growth factor (57)
. Thus, the long-term overexpression of anti-oxidant enzymes and cytoprotective genes in the myocardium provides a strategy for enhanced protection from I/R-induced injury.
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A potential strategy for acute protection from I/R injury may be the inhibition of proinflammatory genes activated by I/R. Morishita et al. (58)
showed that pretreatment with a decoy oligonucleotide capable of inhibiting the trans-activating activity of NF-
B reduces myocardial infarct after coronary artery ligation in rats. Although the rapid in vivo degradation of oligonucleotides precludes their use in long-term myocardial protection, this strategy may be useful in treatment of acute myocardial ischemia in cardiac transplantation (59)
For example, treatment with antisense oligonucleotide directed against intercellular adhesion molecule-1 (ICAM-1) prolongs cardiac allograft tolerance and long-term survival when administered ex vivo before transplantation into the host (60)
. Such an approach could be useful in the preparation of donor hearts for transplantation.
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Gene therapy for myocardial ischemia |
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TOPABSTRACTINTRODUCTIONStrategies for genetic...Targets for gene therapy...Gene therapy for myocardial...Gene therapy for myocardial...Gene therapy for rescue...Gene therapy for inherited...Cell-based therapy for...Tissue engineeringPerspectives and future...REFERENCES |
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, 15
), FGF (40)
and hepatocyte growth factor (HGF) (41)
. In all cases, improvement in tissue perfusion was accompanied by morphological and angiographic evidence of new vessel formation, establishing a relationship between improved tissue viability and neovascularization. For example, Mack et al. (39)
showed improvement in regional myocardial perfusion and left ventricular function in response to stress after intramyocardial delivery of VEGF121 by adenovirus in an ameroid constrictor model of chronic myocardial ischemia in pigs. Using a similar model, Giordano et al. (40)
showed improvement in blood flow and a reduction in stress-induced functional abnormalities after intracoronary injection of an adenovirus vector encoding human FGF-5 as early as 2 wk after placement of the ameroid in the proximal left circumflex artery. The improved blood flow was attributed to an increase in capillary to fiber ratios.
The success of these preclinical studies led to several small-scale nonrandomized phase I and II clinical trials with patients suffering from myocardial ischemia (20, 61, 62, 64–65; for a review, see ref 66
). These safety trials, although consisting of small nonrandomized patient samples have demonstrated the potential of angiogenic gene therapy for treatment of ischemic heart disease. Losordo et al. (20)
carried out a phase I study in five male patients aged 53–71 years of age with CAD who did not respond to conventional anti-anginal therapy. The authors reported that direct intramyocardial delivery of naked plasmid encoding VEGF165 into the ischemic myocardium led to reduction of anginal symptoms and improvement, albeit modest, in left ventricular function concomitant with reduced ischemia. Rosengart and colleagues (61)
reported significant improvement in regional ventricular function and wall motion in the region of vector administration after intramyocardial delivery of VEGF121 in patients undergoing conventional coronary artery bypass grafting compared with patients receiving placebo. Using catheter-based delivery of naked VEGF165 assisted by electromechanical NOGA mapping of the left ventricle in patients with chronic myocardial ischemia, Vale and colleagues (64)
reported significant reductions in weekly anginal attacks for as long as 1 year after gene delivery in the treated patients compared the patients receiving placebo. Recently, the results of the angiogenic gene therapy (AGENT) double-blinded, randomized, placebo-controlled trial using dose-escalating adenovirus-mediated intracoronary delivery of FGF-4 in patients with angina showed increased exercise tolerance and improved stress echocardiograms 4 and 12 wk after gene transfer in the patients that received FGF-4 gene therapy compared with patients receiving placebo (65)
. Unfortunately the outcome beyond 12 wk has not been reported.
Despite the promising findings of these small-scale trials, there are several outstanding issues relating to the safety and sustainability of the approach. The safety of therapeutic angiogenesis requires systematic evaluation. This is significant in light of recent evidence that constitutive overexpression of VEGF in mouse heart led to intramural angiomas, followed by heart failure and death (67)
, and may accelerate plaque progression in atherosclerotic vessels (68)
. This observation underscores the necessity for regulated expression of proangiogenic factors. Such a strategy may require the incorporation of promoter sequences such as hypoxia-sensitive responsive elements capable of rendering expression of the therapeutic transgene subservient to the pathophysiological changes in myocardial oxygen tension. This concept has been validated by Su et al. (69)
, who demonstrated that VEGF expression by an AAV vector in ischemic myocardium could be regulated by hypoxia by incorporation of the erythropoietin responsive element (HRE). Another approach to achieve regulated therapeutic angiogenesis uses engineered transcription factors capable of activating endogenous VEGF expression as a strategy to induce VEGF expression in pathophysiological conditions (70)
. These novel strategies may allow endogenous regulation of angiogenesis so that the magnitude of neovascularization is matched to the severity of the ischemic insult. More work is necessary to determine the safest and most efficacious route and method of therapeutic gene delivery in order to avert potentially hazardous late onset side effects such as neovascularization of occult neoplasms or peripheral vascular effects that may result in edema and hypotension. The optimal strategy may require targeted tissue delivery by incorporation of cell-specific promoters for expression of the transgene exclusively at the target sites. Regarding the therapeutic sustainability, it is necessary to establish whether the desired long-term therapeutic effect can be achieved with a single administration of the therapeutic gene or whether multiple treatments may be required. This is an important aspect because VEGF-induced neovessels tend to regress soon after termination of transgene expression in the absence of adequate blood supply (71)
.
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Gene therapy for rescue of contractile function |
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TOPABSTRACTINTRODUCTIONStrategies for genetic...Targets for gene therapy...Gene therapy for myocardial...Gene therapy for myocardial...Gene therapy for rescue...Gene therapy for inherited...Cell-based therapy for...Tissue engineeringPerspectives and future...REFERENCES |
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, leading to decreased contractility. ß-Adrenergic receptors (ß-AR) are G-protein-coupled receptors that play an essential role in regulation of myocardial contractility and inotropic state in response to neurohumoral stimulation (73)
. The ß-AR signaling and calcium regulating pathways have for several years been used as targets for treatment of heart failure (72)
. Recent preclinical studies suggest that genetic manipulation of these therapeutic targets may be a viable strategy for treatment of heart failure (Table 2)
. Adenovirus-mediated intracoronary delivery of the ß2-AR gene led to improvements in basal and isoproterenol-stimulated LV contractility and hemodynamic function in rabbits (44)
, and rescued ß-AR signaling in ventricular myocytes from failing hearts (13
, 74)
. Similarly, adenovirus delivery of the ß-ARKct peptide inhibitor improved postinfarction LV function significantly in rabbits after myocardial infarction in parallel with increased ß-AR-mediated stimulation of adenylate cyclase activity and cAMP generation (75)
. Recently, Roth et al. (45
, 76)
demonstrated that cardiac-specific overexpression of adenylate cyclase type VI (ACVI) improves ventricular function, restoring ß-AR-stimulated cAMP generation and increasing long-term survival in mice rendered cardiomyopathic by overexpression of Gq protein. These findings suggest that gene transfer protocols aimed at normalizing ß-AR signaling may have application as a therapeutic strategy for functional rescue of the failing heart. For example, exogenous overexpression of ß-AR receptors and signaling proteins by gene transfer could be used as a strategy to compensate for the decrease in endogenous ß-AR density and sensitivity resulting from chronic sympathetic activation in heart failure, thereby normalizing left ventricular function.
Gene therapy strategies for normalization of myocardial cytosolic calcium transients have also shown promising results in experimental models of heart failure (Table 2)
(43, 77, 78; for a review, see ref 79
). The ratio of phospholamban to SERCA2a is increased in heart failure, resulting in decreased Ca2+ ATPase activity and reduced calcium uptake by the SR (72)
. Adenovirus-mediated overexpression of SERCA2a in neonatal cardiac myocytes enhances contraction by increasing peak [Ca2+]i release and a decrease in resting [Ca2+]i (78)
. In a rat model of heart failure induced by aortic banding, intracoronary SERCA2a gene delivery by adenovirus at approximately the time of transition from compensated hypertrophy to heart failure restored systolic and diastolic function concomitant with an increase in basal Ca2+-ATPase activity (44)
, and improved phosphocreatine/ATP ratio and long-term survival (77)
. SERCA2a gene transfer normalized cytosolic transients and restored contractile function in ventricular myocytes isolated from patients with end-stage heart failure (80)
. Presumably, overexpression of SERCA2a restores the normal stoichiometry between phospholamban and the Ca2+-ATPase, thereby preventing cytosolic calcium overload and left ventricular dysfunction. Conversely, antisense inhibition of phospholamban was shown to improve contractility in ventricular myocytes of end-stage heart failure patients (81)
in association with improved calcium sensitivity of SERCA and reduced time for recovery of the Ca2+ transient.
The long-term efficacy and safety of adenoviral-mediated myocardial expression of adrenergic and calcium-regulating proteins remain to be established. Sustained expression of the therapeutic transgene may be essential for rescue of the failing heart, thus necessitating the use of a vector type capable on long-term transgene expression. Second, the physiological consequences of chronic ß-AR and SERCA2a overexpression are not known. Although transgenic mice with cardiac-specific overexpression of ß2-AR or SERCA2a do not show any morphological evidence of myocardial pathology, it is not known whether viral-mediated expression of these proteins has any secondary effects besides calcium regulation and inotropic state. Concerns have been raised that the increase in SERCA2a expression by gene transfer in the failing heart may impose extra demands on myocardial energy expenditure due to increased inotropic state and may cause adverse electrophysiological events such as arrhythmias (for a review, see ref 82
). Such potential adverse effects could accelerate myocardial cell death and precipitate the progression of heart failure, and will have to be addressed before inotropic gene therapy could be considered for clinical trial.
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Gene therapy for inherited and congenital heart disease |
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TOPABSTRACTINTRODUCTIONStrategies for genetic...Targets for gene therapy...Gene therapy for myocardial...Gene therapy for myocardial...Gene therapy for rescue...Gene therapy for inherited...Cell-based therapy for...Tissue engineeringPerspectives and future...REFERENCES |
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. Kawada et al. (82)
showed that intramyocardial delivery of 
-sarcoglycan to 5-wk-old TO-2 Syrian hamsters using an AAV vector completely rescued the progression of cardiomyopathy and led to a drastic increase in life expectancy. Similarly, Ikeda et al. (83)
showed that coronary retroinfusion of adenovirus vector coding for -sarcoglycan in 8- to 12-wk-old BIO 14.6 hamsters, resulted in restoration of -, 
-, and ß-sarcoglycan to the sarcolemma and improved ventricular function compared with age-matched untreated CM hamsters. Myocardial delivery of genes encoding defective channel proteins or regulatory G-proteins may provide a strategy for correction of the genetic defects associated with inherited and acquired LQT syndromes. Nuss et al. (84)
reported that adenoviral transfer of the human HERG gene to adult rabbit ventricular myocytes maintained in primary culture led to abbreviated action potentials and drastically reduced the incidence of early after-depolarizations after a train of action potentials. This was found to be associated with increased duration of the refractory period. Similarly, Donahue et al. (86) were able to reduce heart rate after atrial fibrillation in pigs by local delivery of Gi2 gene to the atrioventricular node by adenovirus, suggesting this approach may have application in the treatment of atrial arrhythmias.
The design of gene therapies for congenital heart and vessel disease is problematic. The precision, both in time and mechanism, by which these developmentally regulated genes exert their effects on heart morphogenesis and development (87
, 88)
dictates that an external corrective measure such as replacement of defective genes, needs to be performed before the developmental programs affected by the mutated genes are activated, since the anatomical and functional defects emanating from these mutations may be irreversible (88)
. Second, the dependency of normal heart and vessel development and maturation on precise stage-specific regulation of these morphogenetic genes (87)
mandates that corrective strategies be amenable to regulation by the endogenous mechanisms responsible for normal development. The ability to intervene and reprogram a defective gene within the crucial developmental time window requires the availability of diagnostic tools that would permit detection of such mutations before the onset of disease and access to an effective system for in utero gene delivery. The prohibitive costs of screening technologies for congenital diseases currently restrict its use to cases with a strong familial history, leaving many cases undiagnosed.
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Cell-based therapy for myocardial rescue and repair |
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TOPABSTRACTINTRODUCTIONStrategies for genetic...Targets for gene therapy...Gene therapy for myocardial...Gene therapy for myocardial...Gene therapy for rescue...Gene therapy for inherited...Cell-based therapy for...Tissue engineeringPerspectives and future...REFERENCES |
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), express endothelial lineage markers (i.e., CD34+, Flk-1+, VE-cadherin, PECAM-1, von Willebrand factor, eNOS, and E-selectin) (for review, see refs 14
, 90
), and can be expanded and genetically modified ex vivo to yield sufficient numbers for therapeutic applications (Fig. 5
) (10
, 91
92
93)
. The cells, whose abundance is low in basal conditions are recruited to sites of injury, such as ischemic myocardium and damaged blood vessels, where they may participate in local vasculogenesis and repair of the injured vessels (90
, 94
, 95)
. Exogenous administration of cytokines such as VEGF and G-CSF increases the numbers of circulating EPC several-fold by mobilizing them from the bone marrow (10
, 90
, 96
97
98)
.
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The therapeutic potential of EPC for neovascularization and rescue of ischemic myocardium has been demonstrated. Myocardial transplantation of autologous CD31+ cells isolated from peripheral blood induced new vessel formation and improved left ventricular perfusion and performance in pig hearts rendered ischemic by placement of an ameroid constrictor in the circumflex coronary artery (99)
. Likewise, transplantation of whole (93)
or CD34+-selected human mononuclear cells from peripheral blood immediately after acute myocardial infarction in nude rats led to revascularization and repair of the infarcted myocardium, resulting in reduced interstitial fibrosis and improved ventricular function. Favorable results have also been seen with bone marrow-derived mononuclear cells (BM-MNC). Kocher et al. (100)
reported that intravenous delivery of human CD34+ BM-MNC to nude rats with myocardial infarction led to significant neovascularization of the infarcted myocardium, resulting in reduced apoptosis of myocytes in the peri-infarct area, decreased fibrosis and sustained recovery of left ventricular function. Others have reported that implantation of bone marrow-derived Lin–c–kit+ (101)
or side population (SP) cells (102)
in the infarct border resulted in improved left ventricular function in association with new vessel formation.
In addition to EPC, various other cell sources, such as skeletal myoblasts, embryonic and fetal cardiomyoctes, and bone marrow-derived myocyte progenitors have been used for cellular cardiomyoplasty of infarcted myocardium (Table 3
) (for review, see refs 14
, 103
), but the efficacy of these cells in repair myocardium has been inconsistent. The use of an adult self-regenerating autologous source of progenitor cells with the potential for differentiating into cardiomyocytes would appear to be ideal. Mesenchymal cells from the bone marrow stroma of long bones (MSC) may offer a viable option for cellular cardiomyoplasty using autologous cells. These cells exhibit a high degree of plasticity (104)
and can differentiate into functional cardiomyocytes under specific culture conditions (102
, 104
, 105)
. MSC can be induced to differentiate into synchronously beating cardiomyocytes in vitro after treatment of primary cultures of mouse bone marrow with the cytosine analog 5-azacytidine (106
, 107)
. Several groups have provided evidence that the bone marrow mesenchymal cell population may contain cardiomyocyte precursors. Toma and colleagues (108)
showed that human MSC transplanted into the left ventricular wall of immunodeficient mice differentiate into cardiac myocytes without the need for myogenic differentiation before transplantation. Tomita et al. (107)
reported that transplantation of 4-azacytidine-treated bone marrow cells repopulate the scar and significantly improve left ventricular function in cryoinjured rat hearts. Wang et al. (109)
detected several cell types, including cardiomyocytes, endothelial cells, and fibroblasts, within and on the border of the scar 1 month after intracoronary delivery of retrovirally transduced isogenous bone marrow cells to infarcted rat hearts, suggesting that factors emanating from the injured myocardium may induce trans-differentiation of bone marrow progenitors into the various cell types required for regeneration and maintenance of the myocardium.
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More recently, several groups reported evidence of extracardiac progenitors in necropsy specimens of hearts obtained from subjects that had undergone sex-mismatched heart (110
, 111)
or bone marrow transplantation (112)
. Quaini et al. (110)
and Muller et al. (111)
reported the presence of highly proliferating Y chromosome-positive myocytes and vascular cells in myocardial specimens from male recipients that had received hearts from female donors. The recipient-derived cells expressed stem cell-related antigens, including c-kit, MDR1, and Sca-1 (110)
and connected by gap junction with neighboring myocytes (111)
, indicating that these precursor cells develop into functional cardiomyocytes. The bone marrow is a likely source from which these extracardiac precursor cells are mobilized in response to the injury. The cells probably migrate to the injured myocardium in response to locally released cues such as cytokines, where they may home and participate in the repair of the damaged myocardium (for review, see refs 14
, 113
).
In addition to the marrow-derived precursor cells, clusters of highly proliferating primitive cells have been detected in the infarcted myocardium by two independent groups (114–116; for a review, see ref 113
). In a recently published study, Beltrami and colleagues (115)
reported the identification and isolation of a population of undifferentiated lineage negative (Lin–) cells that express stem cell markers such as c-kit (c-kitPos) and stem cell antigen 1 (Sca-1Pos). These cells were found to be clonogenic and self-renewing and capable of differentiating into all myocardial cell types, including cardiomyocytes, endothelial cells, and vascular smooth muscle cells (115)
. Within the clusters, the progenitor cells were found to be at different stages of cardiomyogenic differentiation, reflecting their cardiogenic potential. This was further supported by the ability of early passage cells to induce significant myocardial regeneration and improve ventricular performance after transplantation into infarcted hearts from syngeneic rats (115)
. Recently the same group reported a dramatic 13-fold increase in the abundance of these proliferating stem cells in the myocardium of patients with aortic stenosis (116)
, raising the intriguing specter that the compensatory cardiac enlargement triggered by the stenosis in these patients is due at least in part to hyperplastic growth induced by these proliferating cells. The identification of resident cardiac progenitors may provide an explanation for the controversial early findings by the same group of actively dividing myocytes in the ventricular myocardium of healthy and end-stage heart failure patients (117)
. The mitotic index in explants from patients with ischemic or idiopathic heart failure was several-fold higher than in explants from patients without heart disease, suggesting that the resident precursor cells constitute a cardiac self-repair mechanism for the replacement of damaged or dying myocytes. Such a mechanism could also potentially play a role in renewal of myocytes lost as a result of biological turnover and cellular aging in the normal heart (117)
. However, the regenerative capacity of this self-repair mechanism has been questioned by some groups on the basis that the small number of resident cells and extracardiac progenitors that are capable of migrating to the heart is insufficient to induce effective long-term regeneration of the myocardium (118
, 119)
.
Some controversy remains regarding the relative roles of cardiac stem cell differentiation and fusion to the regenerative process. Whereas Beltrami et al. (115)
found no evidence of cell fusion after transplantation of c-kitPos cardiac progenitors into the infarcted heart, Oh and colleagues (120)
showed roughly equal contributions of differentiation and fusion to myocardial regeneration. Using a Cre-Lox donor/recipient pair of transgenic mice, this group showed that myocardial regeneration resulted equally from differentiation and fusion of the donor cells with the recipient cells after intravenous administration of Sca-1Pos cells isolated from the hearts of -MHC-Cre into Cre-dependent, LacZ-expressing transgenic mice (R26R) with myocardial infarction. Regardless of the nature of the mechanism(s) of myocardial reconstitution, these studies nevertheless advert to the existence of bona fide resident cardiac progenitors and their potential role in cardiac cell renewal and regeneration.
These encouraging preclinical results have led to several recent small-scale feasibility and safety studies to evaluate the therapeutic potential of bone marrow cell transplantation in treatment of ischemic heart disease and myocardial infarction (121
122
123
124
125)
. Strauer et al. (121)
reported that intracoronary delivery of unfractionated autologous mononuclear bone marrow cells 6 days after infarction led to a reduction in infarct size and improvement in ventricular function and chamber geometry 10 wk after transplantation. In a recent small-scale phase I clinical trial, Stamm and colleagues (122)
injected autologous AC133+ bone marrow cells into the infarct border during CABG in 6 patients that had suffered earlier acute transmural myocardial infarction. The authors reported improved perfusion of the infarcted area and significant enhancement of global left ventricular function 3–9 months after surgery. Using a randomized group of 20 patients with reperfused acute MI, Assmus and colleagues (123)
reported that intracoronary infusion of either BM-MNC or PB-MNC 4 days after infarction led to significant improvement in global left ventricular ejection fraction and wall motion in the infarct zone and reduced systolic dimensions at 4 months follow-up, in association with increased coronary flow reserve in the infarct artery and greater viability in the infarct zone. Two other groups have reported that trans-endocardial delivery of autologous BM-MNC using NOGA mapping led to significant improvements in left ventricular perfusion and performance in patients with end-stage ischemic heart disease (124)
or with stable angina (125)
.
Nevertheless, these findings should be considered preliminary. The nature of the mobilizing, migration, and homing signals for bone marrow progenitor cells and the mechanism of differentiation and incorporation into the target tissues need to be identified. Multimember controlled trials are needed in order to define and standardize the optimal time and method of delivery and effect. A significant number of the transplanted cells die or detach soon after transplantation, suggesting that strategies to improve cell survival and adhesion at the time of transplantation may be necessary. We showed recently that genetic modification of the bone marrow mesenchymal cells before grafting with a retroviral vector overexpressing the survival gene Akt1 significantly reduced peri-infarct death of the transplanted cells, leading to reduced myocardial scarring and improved ventricular function (126)
. Finally, the morphological and functional complexity of the myocardium raises a cautionary point against designing overly simplistic grafting protocols and suggests that the optimal grafting procedure for cardiac repair may require more than one cell type—for example, cardiomyocytes, fibroblasts, and endothelial cells—to produce a graft that is able to recapitulate normal cardiac function.
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Tissue engineering |
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TOPABSTRACTINTRODUCTIONStrategies for genetic...Targets for gene therapy...Gene therapy for myocardial...Gene therapy for myocardial...Gene therapy for rescue...Gene therapy for inherited...Cell-based therapy for...Tissue engineeringPerspectives and future...REFERENCES |
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. Advancement in this area is conditioned by the development of immunocompatible biodegradable materials that could be used as scaffolds shaped to the desired configurations and to which autologous cells may be seeded to provide a biologically active surface. The cells may be genetically engineered ex vivo to impart desirable qualities to the grafts, such as an enhanced antithrombotic surface in vascular prosthetic grafts and cardiac valves or the capability to synthesize angiogenic, vasodilatory, or cytoprotective factors for maintenance and survival of the grafts.
Tissue engineering has also been used to produce biologically active autologous heart valves and cardiac grafts (128
, 129)
. The engineering of a complex structure such as a heart valve that needs to perform precise mechanical functions in an environment of high shear stress is challenging, but strides have been made in the last few years in developing of tissue engineered valve replacements. Sodian et al. (128)
have recently reported the construction of a biodegradable trileaflet heart valve in which ovine vascular cells collected from the carotid artery were seeded onto a porous polyhydroxyalkanoate scaffold and allowed to proliferate under pulsatile flow in a bioreactor. Histological examination of the leaflets showed cell proliferation and deposition of matrix. The engineered valves were then implanted in the autologous donors in place of the native pulmonary leaflets and their function was assessed by echocardiography. The functional evaluation revealed normal valve pressure gradients, minimal regurgitation, and the absence of stenosis for up to 17 wk after transplantation, suggesting the potential usefulness of approach for treatment of patients with end-stage valvular heart disease. Seeding of decellularized porcine valves with human fibroblasts is also being investigated for xenografting in humans (129)
, but no functional data are yet available on the effectiveness of this approach.
Attempts have been made to engineer cardiac muscle using various biopolymers for scaffolds (130
131
132)
. Leor et al. (130)
constructed an artifical cardiac graft by seeding fetal cardiac cells into 3-D porous alignate scaffold. After 4 days of culture, aggregates of contracting cells appeared in the 3-D alignate lattice. Subsequent implantation of the graft into rats that had undergone earlier myocardial infarction led to attenuation of LV dilatation and recuperation of ventricular function compared with untreated animals. Postmortem histological examination of the grafts revealed almost complete disappearance of the scaffold, and the presence of myofibers and neoangiogenesis in a lattice of collagen. Using a similar approach, Li and colleagues (131)
impregnated a biodegradable gelatin mesh (Gelfoam) with rat fetal ventricular myocytes. After culture for 7 days, the grafts showed beating activity and were implanted into cryoinjured rat hearts. Histological evaluation of the grafts 5 wk after transplantation showed that the graft became vascularized and that the grafted myocytes formed junctions with the native myocytes. In an attempt to recreate the geometric arrangement of cardiac fibers in the heart, McDevitt et al. (132)
recently seeded neonatal myocytes onto micropatterned laminin-coated Sylgard 184 stamps. The myofibrils of the patterned myocytes aligned parallel to the laminin surface; the fibers expressed N-cadherin and connexin 43, both ends forming structures that ultrastructurally resembled intercalated disks. Narrow patterning of the laminin lanes allowed the formation of gap junctions between adjacent fibers resulting in synchronous beating. The authors suggested that the growth of patterned cultures could be used for accurate reproduction of native myocardial structures.
The field of tissue engineering has taken big strides in the last decade. As advances continue in the development of scaffolding and interfacing technologies, we may envisage the possibility of manufacturing fully functional biocompatible organs that could be used to replace terminally diseased organs in the absence of suitable donors. Such a development would overcome the problems of availability and accessibility that patients in need of a life-saving transplant often face today. The ability to produce custom-made organs constructed to meet exact specifications may have entered the realm of reality with the advances in these developing technologies. Clearly, the way forward in this burgeoning field will require a collective multidisciplinary effort in order to address the very complex physiological, medical, and design issues associated with the construction of such artificial organs.
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Perspectives and future directions |
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TOPABSTRACTINTRODUCTIONStrategies for genetic...Targets for gene therapy...Gene therapy for myocardial...Gene therapy for myocardial...Gene therapy for rescue...Gene therapy for inherited...Cell-based therapy for...Tissue engineeringPerspectives and future...REFERENCES |
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With regard to the use of progenitor cell for myocardial rescue and repair, there is a pressing need to define and standardize the optimal conditions for therapeutic application, specifically, the timing, route, and method of delivery and purity of the cells used in transplantation protocols. Furthermore, the long-term safety and efficacy of the approach need to be evaluated, amid potential concerns about delayed onset complications such as visualization of occult neoplasias, development of age or diabetic-induced retinopathies or the appearance of ectopic foci in the myocardium that could lead to life-threatening arrhythmias. The ideal molecular therapy for myocardial infarction may require a combination of cell transplantation and proantigenic gene therapy for long-term maintenance of the regenerated myocardium. Due to regulatory hurdles, such potentially synergistic combinatorial approaches have seldom been considered in the design of cardiovascular gene therapy strategies that have traditionally been developed around a single therapeutic target.
Finally, we see future advances in gene and cell therapies linked to genomic research. Genomic profiling and screening are being used for molecular phenotyping of patients and will permit the detection of disease-causing polymorphisms and the design of individualized therapies. The convergence of gene transfer technology and genomic technology will help reveal the function of new genes and may help uncover new roles for previously known genes which could lead to the discovery of new therapeutic targets.
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ACKNOWLEDGMENTS |
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Received for publication October 30, 2003. Accepted for publication December 18, 2003.
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REFERENCES |
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TOPABSTRACTINTRODUCTIONStrategies for genetic...Targets for gene therapy...Gene therapy for myocardial...Gene therapy for myocardial...Gene therapy for rescue...Gene therapy for inherited...Cell-based therapy for...Tissue engineeringPerspectives and future...REFERENCES |
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B binding sites prevents myocardial infarction. Nat. Med. 3,894-899[CrossRef][Medline]
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