Figure 5. Impairment by glutathione of the decrease of cellular glutathione content induced by 7-ketocholesterol. Glutathione (GSH) content was measured by flow cytometry with monochlorobimane at 30 min, 6 h, 12 h, 18 h, and 24 h in untreated and GSH-treated cells, and at 6, 12, 18, and 24 h in 7-ketocholesterol and (7-ketocholesterol plus GSH)-treated cells. GSH (10 mmol/l) was added in the culture 30 min before 7-ketocholesterol (40 µg/ml). At the end of the indicated incubation times, the cellular GSH content was quantified by flow cytometry after staining with monochlorobimane (A) and the proportion of monochlorobimane-positive cells was determined (B). Data are mean ±SEM of four independent experiments. Significance of the differences between control and 7-ketocholesterol-treated cells (*P<0.05); significance of the differences between 7-ketocholesterol-treated cells and (7-ketocholesterol + glutathione)-treated cells (**P<0.05).
