Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 - (igSup1.tif; 4.0 MB) Toll-like receptor 2 (TLR2) mRNA expression two days after the cerebral injection of ethidium bromide and LPS. Representative examples in coronal sections hybridized with TLR2 cRNA antisense probe and exposed to x-ray films (Kodak, Biomax). The figure depicts the robust induction of the innate immune transcript following LPS infusion (2.5 μg). Ethidium bromide (EtBr – 0.1% solution) had by itself a very modest effect on TLR2 gene expression, but it caused a robust and widespread microglial activation when combined with the endotoxin.
• Supplemental Figure 2 - (igSup2.tif; 4.37 MB) Maintained activation of the gene encoding toll-like receptor 2 (TLR2) 5 days following acute demyelination with ethidium bromide (EtBr) and lipopolysaccharide (LPS) administration. Representative signals in coronal sections hybridized with TLR2 cRNA antisense probe and exposed to x-ray films. Please note the robust and localized activation at the lesion site (EtBr section). The widespread distribution of the transcript can still be detected 5 days post EtBr (0.05%)/LPS (1.0 μg) injection.