S-glutathiolation by peroxynitrite of p21ras at cysteine-118 mediates its direct activation and downstream signaling in endothelial cells FASEB J. Clavreul et al.
10.1096/fj.05-4875fje
Supplemental Data
Files in this Data Supplement:
Supplemental Figures A-F
-
(ClavreulSupplementalFigures.ppt; 344 KB) A) Biotin-tagged and total GSH pools after supplementation with biotinylated GSH ester. B) Pathways not involved in peroxynitrite-induced signaling. C) Lack of role of eNOS or NADPH oxidase in peroxynitrite-induced signaling. D) Oxidation of p21ras is reversed by DTT as demonstrated with HPDP labeling. E) Raf-1 binds to p21ras only when it is bound to biotin-tagged GSH after stimulation with peroxynitrite. F) DTT but not ascorbate reverses biotin-tagged GSH binding to p21ras.
