Supplemental Figures I–IV
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(WadaSupplemental.zip; 486 KB)
Figure I: Densitometry was used to calculate the ratio of target genes and β-actin signals shown in Figure 1 of the manuscript. The results are expressed as fold induction relative to β-actin. The data represent the mean and standard error values from 3 independent experiments. *, P<0.05 compared to control non-preconditioned (NP) cells. Glu, high glucose; heat; hyperthermia; Oxy, hypoxia; TNF, TNF-α.
Figures II: Northern blot analysis of VCAM-1 mRNA in HUVEC preconditioned for 14 h in the absence (C) or presence of 1.5 U/ml thrombin (Th), 10 ng/ml TNF-α (Tn), LPS, 50 ng/ml vascular endothelial growth factor (V) or 100 ng/ml insulin-like growth factor (I), and then incubated with (+) or without (–) 1.5 U/ml thrombin for 4 h. Results are representative of 3 independent experiments.
Figures III: A) Colormap of signal values for the genes in Group A. B) Colormap of signal values for the genes in Group C. Gene names and Affymetrix IDs are shown next to each row. For each gene, red and blue indicate high and low expression respectively. For visual purposes the signal values are normalized to the range [–3,3]. C) biological process categorization of the genes in group A. D) biological process categorization of the genes in group B. E) biological process categorization of the genes in group C.
Figures IV: Densitometry was used to calculate the ratio of target genes and β-actin signals shown in Figure 8 of the manuscript, and described above in Supplemental Fig. I.
