Hypoxia up-regulates expression of Eph receptors and ephrins in mouse skin FASEB J. Vihanto et al.
10.1096/fj.04-3647fje
Supplemental Data
Files in this Data Supplement:
Supplemental Figure 1
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(SupplementalFig1.ppt; 38.8 MB) EphB4 (A), ephrinB2 (B), EphA2 (C), ephrinA1 (D) and control with non-immune rabbit serum (E) protein expression (brown signal) shown by immunohistochemistry analysis in normal skin (1), 24 hours (2) and 48 hours after (3) exposure to hypoxia (100× magnification), illustrating the localization of the proteins of interest. Counterstaining with hematoxylin and development with DAB. Vessels are indicated with arrowheads.
Supplemental Figure 2
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(SupplementalFig2.ppt; 9.2 MB) HIF-1α (A) and VEGF (B) protein expression (green signal) shown by immunofluorescence analysis in Hep3B cells (HIF-1α) or in PC-3 cells (VEGF). 1= no addition, 2= CoCl2 treatment for 8 hours and 3= transfection with siHIF-1α with CoCl2 treatment for 8 hours. 400× magnification.
Supplemental Figure 3
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(SupplementalFig3.ppt; 15.7 MB) EphB4 (A), ephrinB2 (B), EphA2 (C) and ephrinA1 (D) protein expression (red signal) shown by immunofluorescence analysis in Hep3B cells (EphB4 and ephrinB2) or in PC-3 cells (EphA2 and ephrinA1). 1= no addition, 2= CoCl2 treatment for 8 hours and 3= transfection with siHIF-1α with CoCl2 treatment for 8 hours. 400× magnification.
