Supplemental Data

Files in this Data Supplement:

• Supplemental Movie 1 - (YamashitaSupplementalMovie1.mov; 2 MB) Spontaneously beating colonies observed at day 5 of Flk1⁺ cell culture on OP9 cells (Flk-d5) (Figure 2A).
• Supplemental Movie 2 - (YamashitaSupplementalMovie2.mov; 3 MB)Cavity-like formation of spontaneously beating cells on OP9 cells (Flk-d7) (Figure 2B).
• Supplemental Movie 3 - (YamashitaSupplementalMovie3.mov; 2.75 MB) Network-like structure of spontaneously beating cells on OP9 (Flk-d14) (Figure 2C).
• Supplemental Movie 4 - (YamashitaSupplementalMovie4.mov; 1.8 MB) GFP⁺ beating colony on OP9 from ES cells carrying αMHC promoter-driven GFP gene (Figure 2D).
• Supplemental Movie 5 - (YamashitaSupplementalMovie5.mov; 3 MB) Purified cardiomyocytes spontaneously and synchronously beating on type I collagen gel (Figure 2F).
• Supplemental Movie 6 - (YamashitaSupplementalMovie6.mov; 2.2 MB) Induction of various cardiac cell types from Flk1⁺ cells. Among three spontaneously beating colonies observed, the population on the lower left is a "pacemaking" colony. Contraction starts from the lower left colony and is conducted in an upper right direction. Delay of contraction between colonies suggests the existence of the conduction system with node-like structure among induced cells. Thus, pacemaker, ventricular, and conduction system cells should be induced from Flk1⁺ cells on OP9 cells.
• Supplemental Movie 7 - (YamashitaSupplementalMovie7.mov; 2 MB) Spontaneously beating cells from a single Flk1⁺ cells on OP9 (Flk-d6).
• Supplemental Movie 8 - (YamashitaSupplementalMovie8.mov; 2.3 MB) Spontaneously beating cells on OP9 from embryonic FCV cells obtained from E8.0–8.25 mouse embryos (Day 3).