Geranylgeranyl transferase inhibition stimulates antimelanoma immune response through MHC class I and costimulatory molecule expression FASEB J. Tilkin-Mariamé et al.
10.1096/fj.04-3482fje
Supplemental Figure 1
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(Fig1Supp.ppt; 29 KB)Sup Inhibition of enzymatic activities by FTI-277 and GGTI-298 were tested on proteins extracts from B16F10 treated cells. Western-blot analyses were performed with 30 μg of protein per sample for the farnesylation of HDJ-2 after 4 days of FTI-277 (5 or 10 μM) treatment and for the geranylgeranylation of Rap-1A after 4 days of GGTI-298 (5 or 10 μM) treatment. Data are from one representative experiment of 2 additional experiments.
Supplemental Figure 2
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(Fig2Supp.ppt; 24 KB)Sup Capacity of in vitro growing back of treated B16F10 cells grow. After 4 days of in vitro treatment, tumor cell cycles were analyzed by flow cytometry according Vindelov protocol. Data represent the means and standard deviations of 3 independent experiments.
