Supplemental Data

Files in this Data Supplement:

• Supplemental Files - (SwartzSupplementalfiles.ppt; 117KB)

  Supplemental Fig. 1: Binding of scFv-Clones to recombinant CHO cells. Flow cytometric histograms of scFvs (2 μg/ml) binding on CHO cells expressing wild type α_IIbβ₃ (gray histograms) and "activated" (VGFFK-deleted) α_IIbβ₃ (white histograms) CHO cells for three representative clones (MA2, SCE5 and MA4).

  Supplemental Fig. 2: Expression level of CHO cells transfected with wild type α_IIbβ₃ ("wild type" CHO cells) and VGFFK-deleted and thus activated _IIbβ₃ ("VGGFK-deleted" CHO cells). Flow cytometric histograms as obtained with specific antibodies against the two subunits of α_IIbβ₃, CD 41 (second row) and CD61 (third row) and an isotypic control (first row).

  Supplemental Fig. 3: Exclusion of crossreactivity with other integrins. Depicted are the histograms obtained in flow cytometry with scFv clones MA4, MA2 and SCE5 (2 μg/ml) on recombinant CHO cells expressing "activated" (VGFFK-deleted) Mac-1 and "activated" (VGFFK-deleted) vitronectin receptor α_Vβ₃.