| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The FASEB Journal, Vol 9, 626-635, Copyright © 1995 by The Federation of American Societies for Experimental Biology
REVIEWS |
E Levy, M Mehran and E Seidman
Hopital Sainte-Justine, Department of Nutrition, Universite de Montreal, Quebec, Canada.
Caco-2 cells, an intestinal cell line derived from a human colorectal carcinoma that spontaneously differentiates under standard culture conditions, lends itself to the in vitro study of human gut in view of its efficient intestinal transport processes. Among its multiple biological functions are those related to the absorption, transport, and metabolism of lipids and lipoproteins. Despite their intestinal origin, confluent Caco-2 cell monolayers primarily express L-FABP for the uptake of apical dietary long chain fatty acids, incorporating them into triglycerides by the glycerol 3-phosphate pathway, and assembling very-low-density lipoprotein, high-density lipoprotein, and low-density lipoprotein. The monoacyl-glycerol pathway is inactive in Caco-2 cells. Furthermore, the secretion of newly synthesized triglyceride-rich lipoproteins is very restricted, despite abundant production of apolipoprotein (apo) B. The regulation of apoB synthesis and its mRNA editing at the enterocyte level has been intensively examined in Caco-2 cells. Luminal fatty acids, calcium ion, as well as vitamins and hormones are known to modulate the apoB-48/apoB-100 at the transcriptional and/or translational level. The regulation of 3-hydroxy- 3-methylglutaryl-CoA reductase and acyl-CoA: (cholesterol acyltransferase), the key enzymes governing intracellular cholesterol handling, have also been extensively examined in Caco-2 cells. In many respects this cell line provides an excellent in vitro model for the investigation of intestinal lipoprotein metabolism; however, their limited secretion capacity remains a potential drawback to comparisons with the in vivo physiological state.
This article has been cited by other articles:
|
|
 |
|
  A. K. Hihi, M.-C. Beauchamp, R. Branicky, A. Desjardins, I. Casanova, M.-P. Guimond, M. Carroll, M. Ethier, I. Kianicka, K. McBride, et al. Evolutionary conservation of drug action on lipoprotein metabolism-related targets J. Lipid Res., January 1, 2008; 49(1): 74 - 83. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Li, P. N. Black, A. Chokshi, A. Sandoval-Alvarez, R. Vatsyayan, W. Sealls, and C. C. DiRusso High-throughput screening for fatty acid uptake inhibitors in humanized yeast identifies atypical antipsychotic drugs that cause dyslipidemias J. Lipid Res., January 1, 2008; 49(1): 230 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-M. Lo, B. K. Nordskog, A. M. Nauli, S. Zheng, S. B. vonLehmden, Q. Yang, D. Lee, L. L. Swift, N. O. Davidson, and P. Tso Why does the gut choose apolipoprotein B48 but not B100 for chylomicron formation? Am J Physiol Gastrointest Liver Physiol, January 1, 2008; 294(1): G344 - G352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Grenier, F. S. Maupas, J.-F. Beaulieu, E. Seidman, E. Delvin, A. Sane, E. Tremblay, C. Garofalo, and E. Levy Effect of retinoic acid on cell proliferation and differentiation as well as on lipid synthesis, lipoprotein secretion, and apolipoprotein biogenesis Am J Physiol Gastrointest Liver Physiol, December 1, 2007; 293(6): G1178 - G1189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Anwar, J. Iqbal, and M. M. Hussain Mechanisms involved in vitamin E transport by primary enterocytes and in vivo absorption J. Lipid Res., September 1, 2007; 48(9): 2028 - 2038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Sane, D. Sinnett, E. Delvin, M. Bendayan, V. Marcil, D. Menard, J.-F. Beaulieu, and E. Levy Localization and role of NPC1L1 in cholesterol absorption in human intestine J. Lipid Res., October 1, 2006; 47(10): 2112 - 2120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Anwar, H. J. Kayden, and M. M. Hussain Transport of vitamin E by differentiated Caco-2 cells J. Lipid Res., June 1, 2006; 47(6): 1261 - 1273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Peretti, C. C. Roy, E. Drouin, E. Seidman, P. Brochu, G. Casimir, and E. Levy Abnormal intracellular lipid processing contributes to fat malabsorption in cystic fibrosis patients Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G609 - G615. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Murota and J. Storch Uptake of Micellar Long-Chain Fatty Acid and sn-2-Monoacylglycerol into Human Intestinal Caco-2 Cells Exhibits Characteristics of Protein-Mediated Transport J. Nutr., July 1, 2005; 135(7): 1626 - 1630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Deforges, A. Evlashev, M. Perret, M. Sodoyer, S. Pouzol, J.-Y. Scoazec, B. Bonnaud, O. Diaz, G. Paranhos-Baccala, V. Lotteau, et al. Expression of hepatitis C virus proteins in epithelial intestinal cells in vivo J. Gen. Virol., September 1, 2004; 85(9): 2515 - 2523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cai, E. R. M. Eckhardt, W. Shi, Z. Zhao, M. Nasser, W. J. S. de Villiers, and D. R. van der Westhuyzen Scavenger receptor class B type I reduces cholesterol absorption in cultured enterocyte CaCo-2 cells J. Lipid Res., February 1, 2004; 45(2): 253 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Levy, D. Menard, I. Suc, E. Delvin, V. Marcil, L. Brissette, L. Thibault, and M. Bendayan Ontogeny, immunolocalisation, distribution and function of SR-BI in the human intestine J. Cell Sci., January 15, 2004; 117(2): 327 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bernotti, E. Seidman, D. Sinnett, S. Brunet, S. Dionne, E. Delvin, and E. Levy Inflammatory reaction without endogenous antioxidant response in Caco-2 cells exposed to iron/ascorbate-mediated lipid peroxidation Am J Physiol Gastrointest Liver Physiol, November 1, 2003; 285(5): G898 - G906. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zoltowska, E. Ziv, E. Delvin, D. Sinnett, R. Kalman, C. Garofalo, E. Seidman, and E. Levy Cellular Aspects of Intestinal Lipoprotein Assembly in Psammomys Obesus: A Model of Insulin Resistance and Type 2 Diabetes Diabetes, October 1, 2003; 52(10): 2539 - 2545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Marcil, E. Delvin, C. Garofalo, and E. Levy Butyrate Impairs Lipid Transport by Inhibiting Microsomal Triglyceride Transfer Protein in Caco-2 Cells J. Nutr., July 1, 2003; 133(7): 2180 - 2183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Elimrani, K. Lahjouji, E. Seidman, M.-J. Roy, G. A. Mitchell, and I. Qureshi Expression and localization of organic cation/carnitine transporter OCTN2 in Caco-2 cells Am J Physiol Gastrointest Liver Physiol, May 1, 2003; 284(5): G863 - G871. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Singh, O. A. Batuman, H. O. Akman, M. H. Kedees, V. Vakil, and M. M. Hussain Differential, Tissue-specific, Transcriptional Regulation of Apolipoprotein B Secretion by Transforming Growth Factor beta J. Biol. Chem., October 11, 2002; 277(42): 39515 - 39524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Marcil, E. Delvin, E. Seidman, L. Poitras, M. Zoltowska, C. Garofalo, and E. Levy Modulation of lipid synthesis, apolipoprotein biogenesis, and lipoprotein assembly by butyrate Am J Physiol Gastrointest Liver Physiol, August 1, 2002; 283(2): G340 - G346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. PLAT and R. P. MENSINK Increased intestinal ABCA1 expression contributes to the decrease in cholesterol absorption after plant stanol consumption FASEB J, August 1, 2002; 16(10): 1248 - 1253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. L. Black, H. M. Roche, and M. J. Gibney Chronic but Not Acute Treatment with Conjugated Linoleic Acid (CLA) Isomers (trans-10, cis-12 CLA and cis-9, trans-11 CLA) Affects Lipid Metabolism in Caco-2 Cells J. Nutr., August 1, 2002; 132(8): 2167 - 2173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Murota, S. Shimizu, S. Miyamoto, T. Izumi, A. Obata, M. Kikuchi, and J. Terao Unique Uptake and Transport of Isoflavone Aglycones by Human Intestinal Caco-2 Cells: Comparison of Isoflavonoids and Flavonoids J. Nutr., July 1, 2002; 132(7): 1956 - 1961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Levy, D. Menard, E. Delvin, S. Stan, G. Mitchell, M. Lambert, E. Ziv, J. C. Feoli-Fonseca, and E. Seidman The Polymorphism at Codon 54 of the FABP2 Gene Increases Fat Absorption in Human Intestinal Explants J. Biol. Chem., October 19, 2001; 276(43): 39679 - 39684. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V.T. Lobo, L. Huerta, N. Ruiz-Velasco, E. Teixeiro, P. de la Cueva, A. Celdran, A. Martin-Hidalgo, M. A. Vega, and R. Bragado Localization of the Lipid Receptors CD36 and CLA-1/SR-BI in the Human Gastrointestinal Tract: Towards the Identification of Receptors Mediating the Intestinal Absorption of Dietary Lipids J. Histochem. Cytochem., October 1, 2001; 49(10): 1253 - 1260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Morton, V. Nunes, L. Izem, and E. Quintao Markedly Elevated Lipid Transfer Inhibitor Protein in Hypercholesterolemic Subjects Is Mitigated by Plasma Triglyceride Levels Arterioscler. Thromb. Vasc. Biol., October 1, 2001; 21(10): 1642 - 1649. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-Y. Ho and J. Storch Common mechanisms of monoacylglycerol and fatty acid uptake by human intestinal Caco-2 cells Am J Physiol Cell Physiol, October 1, 2001; 281(4): C1106 - C1117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, S. Lu, J. Du, Y. Yao, H. M. Berschneider, and D. D. Black Regulation of apolipoprotein secretion by long-chain polyunsaturated fatty acids in newborn swine enterocytes Am J Physiol Gastrointest Liver Physiol, June 1, 2001; 280(6): G1137 - G1144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Levy, S. Stan, C. Garofalo, E. E. Delvin, E. G. Seidman, and D. Menard Immunolocalization, ontogeny, and regulation of microsomal triglyceride transfer protein in human fetal intestine Am J Physiol Gastrointest Liver Physiol, April 1, 2001; 280(4): G563 - G571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Courtois, I. Suc, C. Garofalo, M. Ledoux, E. Seidman, and E. Levy Iron-ascorbate alters the efficiency of Caco-2 cells to assemble and secrete lipoproteins Am J Physiol Gastrointest Liver Physiol, July 1, 2000; 279(1): G12 - G19. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sonoyama, K. Suzuki, and T. Kasai Peptide YY Stimulates the Expression of Apolipoprotein A-IV Gene in Caco-2 Intestinal Cells Experimental Biology and Medicine, March 1, 2000; 223(3): 270 - 275. [Abstract] [Full Text]
|
|
|
|
|
|
W. Liao and L. Chan Apolipoprotein B, a Paradigm for Proteins Regulated by Intracellular Degradation, Does Not Undergo Intracellular Degradation in CaCo2 Cells J. Biol. Chem., February 11, 2000; 275(6): 3950 - 3956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Levy, J.-F. Beaulieu, E. Delvin, E. Seidman, W. Yotov, J.-R. Basque, and D. Ménard Human crypt intestinal epithelial cells are capable of lipid production, apolipoprotein synthesis, and lipoprotein assembly J. Lipid Res., January 1, 2000; 41(1): 12 - 22. [Abstract] [Full Text]
|
|
|
|
|
|
J. Luchoomun and M. M. Hussain Assembly and Secretion of Chylomicrons by Differentiated Caco-2 Cells. NASCENT TRIGLYCERIDES AND PREFORMED PHOSPHOLIPIDS ARE PREFERENTIALLY USED FOR LIPOPROTEIN ASSEMBLY J. Biol. Chem., July 9, 1999; 274(28): 19565 - 19572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Hamilton, A. Moorehouse, S. R. Lear, J. S. Wong, and S. K. Erickson A rapid calcium precipitation method of recovering large amounts of highly pure hepatocyte rough endoplasmic reticulum J. Lipid Res., June 1, 1999; 40(6): 1140 - 1147. [Abstract] [Full Text]
|
|
|
|
|
|
H. Wang, R. Roberson, J. Du, J. K. Eshun, H. M. Berschneider, and D. D. Black Regulation of apolipoprotein secretion by biliary lipids in newborn swine intestinal epithelial cells Am J Physiol Gastrointest Liver Physiol, February 1, 1999; 276(2): G353 - G362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Spalinger, E. G. Seidman, G. Lepage, D. Menard, V. Gavino, and E. Levy Uptake and metabolism of structured triglyceride by Caco-2 cells: reversal of essential fatty acid deficiency Am J Physiol Gastrointest Liver Physiol, October 1, 1998; 275(4): G652 - G659. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Homan and K. L. Hamelehle Phospholipase A2 relieves phosphatidylcholine inhibition of micellar cholesterol absorption and transport by human intestinal cell line Caco-21 J. Lipid Res., June 1, 1998; 39(6): 1197 - 1209. [Abstract] [Full Text]
|
|
|
|
|
|
M. M. J. van Greevenbroek, M. G. Robertus-Teunissen, D. W. Erkelens, and T. W. A. de Bruin Participation of the microsomal triglyceride transfer protein in lipoprotein assembly in Caco-2 cells: interaction with saturated and unsaturated dietary fatty acids J. Lipid Res., January 1, 1998; 39(1): 173 - 185. [Abstract] [Full Text]
|
|
|
|
|
|
J. Luchoomun, Z. Zhou, A. Bakillah, H. Jamil, and M. M. Hussain Assembly and Secretion of VLDL in Nondifferentiated Caco-2 Cells Stably Transfected With Human Recombinant ApoB48 cDNA Arterioscler. Thromb. Vasc. Biol., November 1, 1997; 17(11): 2955 - 2963. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Hayashi, M. Gohda, S. Matzno, Y. Kubo, H. Kido, T. Yamauchi, and N. Nakamura Possible Mechanism of Action of AE0047, a Calcium Antagonist, on Triglyceride Metabolism J. Pharmacol. Exp. Ther., August 1, 1997; 282(2): 882 - 890. [Abstract] [Full Text]
|
|
|
|
|
|
C. C. Y. Chang, N. Sakashita, K. Ornvold, O. Lee, E. T. Chang, R. Dong, S. Lin, C.-Y. G. Lee, S. C. Strom, R. Kashyap, et al. Immunological Quantitation and Localization of ACAT-1 and ACAT-2 in Human Liver and Small Intestine J. Biol. Chem., September 1, 2000; 275(36): 28083 - 28092. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
