The FASEB Journal, Vol 9, 1362-1370, Copyright © 1995 by The Federation of American Societies for Experimental Biology

RESEARCH COMMUNICATIONS

Deleted in oral cancer-1 (doc-1), a novel oral tumor suppressor gene

R Todd, J McBride, T Tsuji, RB Donoff, M Nagai, MY Chou, T Chiang and DT Wong
Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Boston 02114, USA.

We have identified, isolated, and partially characterized doc-1, a novel cDNA sequence whose activity is consistent with a suppressor of hamster oral carcinogenesis. Doc-1 is an evolutionarily conserved gene exhibiting loss of heterozygosity and marked reduction in expression in malignant hamster oral keratinocytes. The full-length doc-1 cDNA encodes an 87 amino acid product that shows a significant homology to one of the seven novel genes induced in mouse fibroblasts by TNF-alpha. Transfection of the full-length doc-1 cDNA into malignant hamster oral keratinocytes alters the behavior of the recipients in terms of morphology, growth rate, and anchorage-independent growth, suggesting reversion of transformation phenotypes. We propose that doc-1 is a novel tumor suppressor gene in oral cancer development.

This article has been cited by other articles:

				M. L. Figueiredo, Y. Kim, M. A.R. St. John, and D. T.W. Wong p12CDK2-AP1 Gene Therapy Strategy Inhibits Tumor Growth in an In vivo Mouse Model of Head and Neck Cancer Clin. Cancer Res., May 15, 2005; 11(10): 3939 - 3948. [Abstract] [Full Text] [PDF]

				I. Zucchi, E. Mento, V. A. Kuznetsov, M. Scotti, V. Valsecchi, B. Simionati, E. Vicinanza, G. Valle, S. Pilotti, R. Reinbold, et al. Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis PNAS, December 28, 2004; 101(52): 18147 - 18152. [Abstract] [Full Text] [PDF]

				T. S. Kent, Z. Yuan, A. Miller, and T. K. Weber Deleted in Oral Cancer-1 Expression Upregulates Proapoptosis Elements in Microsatellite-Unstable Human Colorectal Cancer Ann. Surg. Oncol., February 1, 2004; 11(2): 192 - 196. [Abstract] [Full Text] [PDF]

				M. G. Hu, G.-F. Hu, Y. Kim, T. Tsuji, J. McBride, P. Hinds, and D. T. W. Wong Role of p12CDK2-AP1 in Transforming Growth Factor-{beta}1-Mediated Growth Suppression Cancer Res., January 15, 2004; 64(2): 490 - 499. [Abstract] [Full Text] [PDF]

				M. E. Terret, C. Lefebvre, A. Djiane, P. Rassinier, J. Moreau, B. Maro, and M.-H. Verlhac DOC1R: a MAP kinase substrate that control microtubule organization of metaphase II mouse oocytes Development, November 1, 2003; 130(21): 5169 - 5177. [Abstract] [Full Text] [PDF]

				M. Shibutani, N. Takahashi, T. Kobayashi, C. Uneyama, N. Masutomi, A. Nishikawa, and M. Hirose Molecular profiling of genes up-regulated during promotion by phenobarbital treatment in a medium-term rat liver bioassay Carcinogenesis, June 1, 2002; 23(6): 1047 - 1055. [Abstract] [Full Text] [PDF]

				R. Todd, P.W. Hinds, K. Munger, A.K. Rustgi, O.G. Opitz, Y. Suliman, and D.T. Wong CELL CYCLE DYSREGULATION IN ORAL CANCER Crit. Rev. Oral. Biol. Med., January 1, 2002; 13(1): 51 - 61. [Abstract] [Full Text] [PDF]

				S. X. Cao, J. M. Dhahbi, P. L. Mote, and S. R. Spindler Genomic profiling of short- and long-term caloric restriction effects in the liver of aging mice PNAS, September 4, 2001; (2001) 191313598. [Abstract] [Full Text] [PDF]

				S. Shintani, M. Mihara, N. Terakado, Y. Nakahara, T. Matsumura, Y. Kohno, H. Ohyama, J. McBride, R. Kent, R. Todd, et al. Reduction of p12DOC-1 Expression Is a Negative Prognostic Indicator in Patients with Surgically Resected Oral Squamous Cell Carcinoma Clin. Cancer Res., September 1, 2001; 7(9): 2776 - 2782. [Abstract] [Full Text] [PDF]

				S. Shintani, H. Ohyama, X. Zhang, J. McBride, K. Matsuo, T. Tsuji, M. G. Hu, G. Hu, Y. Kohno, M. Lerman, et al. p12DOC-1 Is a Novel Cyclin-Dependent Kinase 2-Associated Protein Mol. Cell. Biol., September 1, 2000; 20(17): 6300 - 6307. [Abstract] [Full Text]

				H K Williams Molecular pathogenesis of oral squamous carcinoma Mol. Pathol., August 1, 2000; 53(4): 165 - 172. [Abstract] [Full Text]

				K. MATSUO, S. SHINTANI, T. TSUJI, E. NAGATA, M. LERMAN, J. MCBRIDE, Y. NAKAHARA, H. OHYAMA, R. TODD, and D. T. W. WONG p12DOC-1, a growth suppressor, associates with DNA polymerase {alpha}/primase FASEB J, July 1, 2000; 14(10): 1318 - 1324. [Abstract] [Full Text]

				T. Tsuji, F.-M. Duh, F. Latif, N. C. Popescu, D. B. Zimonjic, J. McBride, K. Matsuo, H. Ohyama, R. Todd, E. Nagata, et al. Cloning, Mapping, Expression, Function, and Mutation Analyses of the Human Ortholog of the Hamster Putative Tumor Suppressor Gene doc-1 J. Biol. Chem., March 20, 1998; 273(12): 6704 - 6709. [Abstract] [Full Text] [PDF]

				S. X. Cao, J. M. Dhahbi, P. L. Mote, and S. R. Spindler Genomic profiling of short- and long-term caloric restriction effects in the liver of aging mice PNAS, September 11, 2001; 98(19): 10630 - 10635. [Abstract] [Full Text] [PDF]