FASEB J. Cell Migration Consortium
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heyward, S. A.
Right arrow Articles by Bautch, V. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heyward, S. A.
Right arrow Articles by Bautch, V. L.

The FASEB Journal, Vol 9, 956-962, Copyright © 1995 by The Federation of American Societies for Experimental Biology

RESEARCH COMMUNICATIONS

Expression and inducibility of vascular adhesion receptors in development

SA Heyward, N Dubois-Stringfellow, R Rapoport and VL Bautch
Department of Biology, University of North Carolina at Chapel Hill 27599, USA.

ICAM-1 and VCAM are cell adhesion receptors expressed on vascular endothelial cells, and their expression is up-regulated as part of the inflammatory response. Tumor-derived Py-4-1 cells were a source of murine endothelial cells, and they showed increased lymphocyte binding when incubated with TNF alpha. Py-4-1 cells stimulated with TNF alpha or LPS also had elevated levels of ICAM-1 and VCAM RNAs, with maximum levels at 2 h. Developmental expression of adhesion receptors was assayed in murine yolk sacs and in cystic embryoid bodies (CEBs) that differentiate in vitro from murine embryonic stem cells. ICAM-1 and VCAM RNAs were expressed in unstimulated yolk sacs and CEBs. Expression was inducible in CEBs by exposure to LPS at all developmental stages, beginning at day 6. The time course of RNA induction in CEBs was similar to that of the Py-4-1 endothelial cell line. ICAM-1 protein expression was localized to vascular and hematopoietic blood islands in the CEBs. These results show that embryonic cells respond to inflammatory mediators by up-regulating expression of vascular adhesion receptors, and they imply an early ontogeny for the endothelial cell signal transduction pathway necessary for leukocyte recruitment.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
L. A. Robinson, C. Nataraj, D. W. Thomas, D. N. Howell, R. Griffiths, V. Bautch, D. D. Patel, L. Feng, and T. M. Coffman
A Role for Fractalkine and Its Receptor (CX3CR1) in Cardiac Allograft Rejection
J. Immunol., December 1, 2000; 165(11): 6067 - 6072.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. D. Redick and V. L. Bautch
Developmental Platelet Endothelial Cell Adhesion Molecule Expression Suggests Multiple Roles for a Vascular Adhesion Molecule
Am. J. Pathol., April 1, 1999; 154(4): 1137 - 1147.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
M. Gawaz, F.-J. Neumann, T. Dickfeld, W. Koch, K.-L. Laugwitz, H. Adelsberger, K. Langenbrink, S. Page, D. Neumeier, A. Schomig, et al.
Activated Platelets Induce Monocyte Chemotactic Protein-1 Secretion and Surface Expression of Intercellular Adhesion Molecule-1 on Endothelial Cells
Circulation, September 22, 1998; 98(12): 1164 - 1171.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
W. Mueller-Klieser
Three-dimensional cell cultures: from molecular mechanisms to clinical applications
Am J Physiol Cell Physiol, October 1, 1997; 273(4): C1109 - C1123.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1995 by The Federation of American Societies for Experimental Biology.