FASEB J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Escargueil-Blanc, I.
Right arrow Articles by Negre-Salvayre, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Escargueil-Blanc, I.
Right arrow Articles by Negre-Salvayre, A.

The FASEB Journal, Vol 8, 1075-1080, Copyright © 1994 by The Federation of American Societies for Experimental Biology

RESEARCH COMMUNICATIONS

Necrosis and apoptosis induced by oxidized low density lipoproteins occur through two calcium-dependent pathways in lymphoblastoid cells

I Escargueil-Blanc, R Salvayre and A Negre-Salvayre
Department of Biochemistry, Faculty of Medicine in Rangueil, University Paul Sabatier, Toulouse, France.

Oxidized low density lipoproteins (LDL) elicit in cultured lymphoblastoid cell lines a delayed and sustained calcium rise followed by a progressive of DNA fragmentation, endogenous proteolysis, and morphological features of necrosis and apoptosis. All these events were blocked by chelating the calcium of the culture medium by EGTA, thus suggesting that the two types of cell death induced by oxidized LDL (necrosis and apoptosis) were subsequent to the rise in calcium. The protease inhibitors leupeptin and antipain were able to block (at least in part) the endogenous proteolysis and the necrotic process, but exhibited no effect on apoptosis and DNA fragmentation. At the opposite, aurintricarboxylic acid and spermine (inhibitors of DNA degradation by endonucleases) inhibit DNA fragmentation and morphological apoptosis, but not endogenous proteolysis and necrosis. These data suggest that cell death induced by oxidized LDL occurs through two calcium-dependent processes triggering 1) on one hand, proteolysis and subsequently necrosis characterized by the loss of cell membrane integrity; and 2) on the other hand, internucleosomal DNA cleavage and subsequently morphological apoptosis. The RNA or protein synthesis inhibitors, actinomycin D and cycloheximide, were completely ineffective in preventing endogenous proteolysis, DNA fragmentation, necrosis, and apoptosis induced by oxidized LDL. The subclassification of the type of apoptosis elicited by oxidized LDL is discussed.


This article has been cited by other articles:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
C. Vindis, M. Elbaz, I. Escargueil-Blanc, N. Auge, A. Heniquez, J.-C. Thiers, A. Negre-Salvayre, and R. Salvayre
Two Distinct Calcium-Dependent Mitochondrial Pathways Are Involved in Oxidized LDL-Induced Apoptosis
Arterioscler. Thromb. Vasc. Biol., March 1, 2005; 25(3): 639 - 645.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
Y. Kashiwakura, M. Watanabe, N. Kusumi, K. Sumiyoshi, Y. Nasu, H. Yamada, T. Sawamura, H. Kumon, K. Takei, and H. Daida
Dynamin-2 Regulates Oxidized Low-Density Lipoprotein-Induced Apoptosis of Vascular Smooth Muscle Cell
Circulation, November 23, 2004; 110(21): 3329 - 3334.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
C. Cuaz-Perolin, C. Furman, G. Larigauderie, L. Legedz, C. Lasselin, C. Copin, M. Jaye, G. Searfoss, K.T. Yu, N. Duverger, et al.
REDD2 Gene Is Upregulated by Modified LDL or Hypoxia and Mediates Human Macrophage Cell Death
Arterioscler. Thromb. Vasc. Biol., October 1, 2004; 24(10): 1830 - 1835.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Maddens, A. Charruyer, I. Plo, P. Dubreuil, S. Berger, B. Salles, G. Laurent, and J.-P. Jaffrezou
Kit signaling inhibits the sphingomyelin-ceramide pathway through PLCgamma 1: implication in stem cell factor radioprotective effect
Blood, July 30, 2002; 100(4): 1294 - 1301.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T.-S. Lee and L.-Y. Chau
Fas/Fas ligand-mediated death pathway is involved in oxLDL-induced apoptosis in vascular smooth muscle cells
Am J Physiol Cell Physiol, March 1, 2001; 280(3): C709 - C718.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J.-P. JAFFREZOU, A. P. BRUNO, A. MOISAND, T. LEVADE, and G. LAURENT
Activation of a nuclear sphingomyelinase in radiation-induced apoptosis
FASEB J, January 1, 2001; 15(1): 123 - 133.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. E. Rusinol, L. Yang, D. Thewke, S. R. Panini, M. F. Kramer, and M. S. Sinensky
Isolation of a Somatic Cell Mutant Resistant to the Induction of Apoptosis by Oxidized Low Density Lipoprotein
J. Biol. Chem., March 15, 2000; 275(10): 7296 - 7303.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
O. VIEIRA, I. ESCARGUEIL-BLANC, G. JÜRGENS, C. BORNER, L. ALMEIDA, R. SALVAYRE, and A. NÈGRE-SALVAYRE
Oxidized LDLs alter the activity of the ubiquitin-proteasome pathway: potential role in oxidized LDL-induced apoptosis
FASEB J, March 1, 2000; 14(3): 532 - 542.
[Abstract] [Full Text]

Home page
 Physiol. Rev.Home page
G. J. Schroepfer Jr.
Oxysterols: Modulators of Cholesterol Metabolism and Other Processes
Physiol Rev, January 1, 2000; 80(1): 361 - 554.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
L. Liu, J. R. Trimarchi, and D. L. Keefe
Thiol Oxidation-Induced Embryonic Cell Death in Mice Is Prevented by the Antioxidant Dithiothreitol
Biol Reprod, October 1, 1999; 61(4): 1162 - 1169.
[Abstract] [Full Text]

Home page
 FASEB J.Home page
N. ANDRIEU-ABADIE, J.-P. JAFFRÉZOU, S. HATEM, G. LAURENT, T. LEVADE, and J.-J. MERCADIER
L-carnitine prevents doxorubicin-induced apoptosis of cardiac myocytes: role of inhibition of ceramide generation
FASEB J, September 1, 1999; 13(12): 1501 - 1510.
[Abstract] [Full Text]

Home page
 J. Lipid Res.Home page
J. Alcouffe, S. Caspar-Bauguil, V. Garcia, R. Salvayre, M. Thomsen, and H. Benoist
Oxidized low density lipoproteins induce apoptosis in PHA-activated peripheral blood mononuclear cells and in the Jurkat T-cell line
J. Lipid Res., July 1, 1999; 40(7): 1200 - 1210.
[Abstract] [Full Text]

Home page
 FASEB J.Home page
O. MEILHAC, I. ESCARGUEIL-BLANC, J.-C. THIERS, R. SALVAYRE, and A. NÈGRE-SALVAYRE
Bcl-2 alters the balance between apoptosis and necrosis, but does not prevent cell death induced by oxidized low density lipoproteins
FASEB J, March 1, 1999; 13(3): 485 - 494.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
I. Escargueil-Blanc, N. Andrieu-Abadie, S. Caspar-Bauguil, R. Brossmer, T. Levade, A. Negre-Salvayre, and R. Salvayre
Apoptosis and Activation of the Sphingomyelin-Ceramide Pathway Induced by Oxidized Low Density Lipoproteins Are Not Causally Related in ECV-304 Endothelial Cells
J. Biol. Chem., October 16, 1998; 273(42): 27389 - 27395.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. Li, B. Yang, and J. L. Mehta
Ox-LDL induces apoptosis in human coronary artery endothelial cells: role of PKC, PTK, bcl-2, and Fas
Am J Physiol Heart Circ Physiol, August 1, 1998; 275(2): H568 - H576.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
I. Suc, O. Meilhac, I. Lajoie-mazenc, J. Vandaele, G. Jürgens, R. Salvayre, and A. Nègre-salvayre
Activation of EGF receptor by oxidized LDL
FASEB J, June 1, 1998; 12(9): 665 - 671.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
N. Auge, I. Escargueil-Blanc, I. Lajoie-Mazenc, I. Suc, N. Andrieu-Abadie, M.-T. Pieraggi, M. Chatelut, J.-C. Thiers, J.-P. Jaffrezou, G. Laurent, et al.
Potential Role for Ceramide in Mitogen-activated Protein Kinase Activation and Proliferation of Vascular Smooth Muscle Cells Induced by Oxidized Low Density Lipoprotein
J. Biol. Chem., May 22, 1998; 273(21): 12893 - 12900.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Harada-Shiba, M. Kinoshita, H. Kamido, and K. Shimokado
Oxidized Low Density Lipoprotein Induces Apoptosis in Cultured Human Umbilical Vein Endothelial Cells by Common and Unique Mechanisms
J. Biol. Chem., April 17, 1998; 273(16): 9681 - 9687.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
C. Bezombes, N. Maestre, G. Laurent, T. Levade, A. Bettaïeb, and J.-p. Jaffrézou
Restoration of TNF-{alpha}-induced ceramide generation and apoptosis in resistant human leukemia KG1a cells by the P-glycoprotein blocker PSC833
FASEB J, January 1, 1998; 12(1): 101 - 109.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
I. Suc, I. Escargueil-Blanc, M. Troly, R. Salvayre, and A. Negre-Salvayre
HDL and ApoA Prevent Cell Death of Endothelial Cells Induced by Oxidized LDL
Arterioscler. Thromb. Vasc. Biol., October 1, 1997; 17(10): 2158 - 2166.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
S. Jovinge, M. Crisby, J. Thyberg, and J. Nilsson
DNA Fragmentation and Ultrastructural Changes of Degenerating Cells in Atherosclerotic Lesions and Smooth Muscle Cells Exposed to Oxidized LDL in Vitro
Arterioscler. Thromb. Vasc. Biol., October 1, 1997; 17(10): 2225 - 2231.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
L. Mabile, O. Meilhac, I. Escargueil-Blanc, M. Troly, M.-T. Pieraggi, R. Salvayre, and A. Negre-Salvayre
Mitochondrial Function Is Involved in LDL Oxidation Mediated by Human Cultured Endothelial Cells
Arterioscler. Thromb. Vasc. Biol., August 1, 1997; 17(8): 1575 - 1582.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
Z. Cai, A. Bettaieb, N. E. Mahdani, L. G. Legres, R. Stancou, J. Masliah, and S. Chouaib
Alteration of the Sphingomyelin/Ceramide Pathway Is Associated with Resistance of Human Breast Carcinoma MCF7 Cells to Tumor Necrosis Factor-alpha -mediated Cytotoxicity
J. Biol. Chem., March 14, 1997; 272(11): 6918 - 6926.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
I. Escargueil-Blanc, O. Meilhac, M.-T. Pieraggi, J.-F. Arnal, R. Salvayre, and A. Negre-Salvayre
Oxidized LDLs Induce Massive Apoptosis of Cultured Human Endothelial Cells Through a Calcium-Dependent Pathway: Prevention by Aurintricarboxylic Acid
Arterioscler. Thromb. Vasc. Biol., February 1, 1997; 17(2): 331 - 339.
[Abstract] [Full Text]

Home page
 Circ. Res.Home page
N. Auge, G. Fitoussi, J.-L. Bascands, M.-T. Pieraggi, D. Junquero, P. Valet, J.-P. Girolami, R. Salvayre, and A. Negre-Salvayre
Mildly Oxidized LDL Evokes a Sustained Ca2+-Dependent Retraction of Vascular Smooth Muscle Cells
Circ. Res., October 1, 1996; 79(4): 871 - 880.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
B. Bjorkerud and S. Bjorkerud
Contrary Effects of Lightly and Strongly Oxidized LDL With Potent Promotion of Growth Versus Apoptosis on Arterial Smooth Muscle Cells, Macrophages, and Fibroblasts
Arterioscler. Thromb. Vasc. Biol., March 1, 1996; 16(3): 416 - 424.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
N. Hilaire, R. Salvayre, J.-C. Thiers, M.-J. Bonnafé, and A. Nègre-Salvayre
The Turnover of Cytoplasmic Triacylglycerols in Human Fibroblasts Involves Two Separate Acyl Chain Length-dependent Degradation Pathways
J. Biol. Chem., November 10, 1995; 270(45): 27027 - 27034.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. J. Scheidegger, R. W. James, and P. Delafontaine
Differential Effects of Low Density Lipoproteins on Insulin-like Growth Factor-1 (IGF-1) and IGF-1 Receptor Expression in Vascular Smooth Muscle Cells
J. Biol. Chem., August 25, 2000; 275(35): 26864 - 26869.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by The Federation of American Societies for Experimental Biology.