| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The FASEB Journal, Vol 7, 964-970, Copyright © 1993 by The Federation of American Societies for Experimental Biology
RESEARCH COMMUNICATIONS |
R Sager, A Anisowicz, M Neveu, P Liang and G Sotiropoulou
Dana-Farber Cancer Institute, Division of Cancer Genetics, Boston, Massachusetts 02115.
A new method of differential expression cloning called differential display (DD) has been used to screen for novel tumor suppressor genes involved in breast cancer. The screen is based on positive selection at the mRNA level for genes expressed in normal mammary epithelial cells but decreased or lost in corresponding tumor cells. A candidate tumor suppressor gene recovered by DD is integrin alpha-6 (alpha 6), a component of the heterodimeric integrin receptors alpha 6 beta 1 and alpha 6 beta 4. Loss of alpha 6 expression was confirmed in total RNAs by Northern blot analysis and by immunostaining with alpha 6 antibodies. Consistent with these cell culture findings, previous immunostaining of mammary tissue sections has identified decreased alpha 6 protein expression during breast tumor progression. Southern blot analysis demonstrated that alpha 6 gene is present in tumor cell lines, suggesting that reexpression may be inducible by pharmacological intervention. The likelihood that alpha 6 may have tumor suppressing activity is supported by growing evidence of a central role for integrins in transducing growth control and differentiation signals from growth factors and the extracellular matrix (ECM).
This article has been cited by other articles:
|
|
 |
|
  F. Stossi, D. H. Barnett, J. Frasor, B. Komm, C. R. Lyttle, and B. S. Katzenellenbogen Transcriptional Profiling of Estrogen-Regulated Gene Expression via Estrogen Receptor (ER) {alpha} or ER{beta} in Human Osteosarcoma Cells: Distinct and Common Target Genes for These Receptors Endocrinology, July 1, 2004; 145(7): 3473 - 3486. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Gudjonsson, L. Ronnov-Jessen, R. Villadsen, F. Rank, M. J. Bissell, and O. W. Petersen Normal and tumor-derived myoepithelial cells differ in their ability to interact with luminal breast epithelial cells for polarity and basement membrane deposition J. Cell Sci., January 1, 2002; 115(1): 39 - 50. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Zajchowski, M. F. Bartholdi, Y. Gong, L. Webster, H.-L. Liu, A. Munishkin, C. Beauheim, S. Harvey, S. P. Ethier, and P. H. Johnson Identification of Gene Expression Profiles That Predict the Aggressive Behavior of Breast Cancer Cells Cancer Res., July 1, 2001; 61(13): 5168 - 5178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ogawa, K. Fukushima, I. Sasaki, and S. Matsuno Identification of genes involved in mucosal defense and inflammation associated with normal enteric bacteria Am J Physiol Gastrointest Liver Physiol, September 1, 2000; 279(3): G492 - G499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. A. Bonass, P. D. Marsh, R. S. Percival, J. Aduse-Opoku, S. A. Hanley, D. A. Devine, and M. A. Curtis Identification of ragAB as a Temperature-Regulated Operon of Porphyromonas gingivalis W50 Using Differential Display of Randomly Primed RNA Infect. Immun., July 1, 2000; 68(7): 4012 - 4017. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-M. Chen, K. L. Schmeichel, I. S. Mian, S. Lelièvre, O. W. Petersen, and M. J. Bissell AZU-1: A Candidate Breast Tumor Suppressor and Biomarker for Tumor Progression Mol. Biol. Cell, April 1, 2000; 11(4): 1357 - 1367. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Kappert, G. Schmidt, G. Doerr, B. Wollert-Wulf, E. Fleck, and K. Graf Angiotensin II and PDGF-BB Stimulate {beta}1-Integrin-Mediated Adhesion and Spreading in Human VSMCs Hypertension, January 1, 2000; 35(1): 255 - 261. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Yang, J. Yu, Z. Luo, C. M. Carthy, J. E. Wilson, Z. Liu, and B. M. McManus Viral Myocarditis : Identification of Five Differentially Expressed Genes in Coxsackievirus B3–Infected Mouse Heart Circ. Res., April 2, 1999; 84(6): 704 - 712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Matsuba, N. Keicho, Y. Higashimoto, S. Granleese, J. C. Hogg, S. Hayashi, and G. P. Bondy Identification of Glucocorticoid- and Adenovirus E1A-regulated Genes in Lung Epithelial Cells by Differential Display Am. J. Respir. Cell Mol. Biol., February 1, 1998; 18(2): 243 - 254. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. M. Miller, R. A. James, J. K. Richer, D. F. Gordon, W. M. Wood, and K. B. Horwitz Progesterone Regulated Expression of Flavin-Containing Monooxygenase 5 by the B-Isoform of Progesterone Receptors: Implications for Tamoxifen Carcinogenicity J. Clin. Endocrinol. Metab., September 1, 1997; 82(9): 2956 - 2961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Sotiropoulou, A. Anisowicz, and R. Sager Identification, Cloning, and Characterization of Cystatin M, a Novel Cysteine Proteinase Inhibitor, Down-regulated in Breast Cancer J. Biol. Chem., January 10, 1997; 272(2): 903 - 910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Traianedes, D. M. Findlay, T. J. Martin, and M. T. Gillespie Modulation of the Signal Recognition Particle 54-kDa Subunit (SRP54) in Rat Preosteoblasts by the Extracellular Matrix J. Biol. Chem., September 8, 1995; 270(36): 20891 - 20894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D Bauer, P Warthoe, M Rohde, and M Strauss Detection and differential display of expressed genes by DDRT-PCR. Genome Res., October 1, 1994; 4(2): S97 - S108. [PDF]
|
|
|
|
 |
|
 Z Zou, A Anisowicz, M. Hendrix, A Thor, M Neveu, S Sheng, K Rafidi, E Seftor, and R Sager Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells Science, January 28, 1994; 263(5146): 526 - 529. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
