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The FASEB Journal, Vol 7, 25-30, Copyright © 1993 by The Federation of American Societies for Experimental Biology

REVIEWS

Self-cleaving catalytic RNA

DM Long and OC Uhlenbeck
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215.

We describe the structures and catalytic properties of several naturally occurring self-cleaving RNA motifs that give 2', 3' cyclic phosphate products. The hammerhead and hairpin motifs are derived from plant pathogenic RNAs and the delta motif is part of the human hepatitis delta element. A fourth motif from Neurospora is less well characterized. By assembling the self-cleaving RNAs from more than one oligoribonucleotide, the cleavage reaction can be examined under a variety of conditions and catalytic turnover can be demonstrated. Mutagenesis and chemical methods to introduce modified nucleotides allowed the structural requirements to be deduced. The role of divalent cations in the catalytic mechanism is discussed.


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Copyright © 1993 by The Federation of American Societies for Experimental Biology.