| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The FASEB Journal, Vol 6, 786-792, Copyright © 1992 by The Federation of American Societies for Experimental Biology
RESEARCH COMMUNICATIONS |
J Alvarez, M Montero and J Garcia-Sancho
Departamento de Bioquimica y Biologia Molecular y Fisiologia, Facultad de Medicina, Universidad de Valladolid, Spain.
The filling state of the intracellular Ca2+ stores of rat thymocytes regulates plasma membrane permeability to Mn2+, used here as a Ca2+ surrogate for plasma membrane Ca2+ channels. Emptying of the Ca2+ stores accelerated Mn2+ entry about 10-fold, and refilling with Ca2+ restored low Mn2+ permeability. The acceleration of Mn2+ entry observed in cells with empty intracellular Ca2+ stores was prevented by cytochrome P450 inhibitors. Imidazole antimycotics, especially econazole and miconazole, were the most potent inhibitors (IC50 approximately equal to 10(-6) M). The inhibitor sensitivity profile was similar to IA-type cytochrome P450. Calmodulin antagonists increased the plasma membrane permeability to Mn2+ in cells with filled Ca2+ stores, and this effect was also blocked by imidazole antimycotics. On this basis, we propose a model in which activation of a cytochrome P450, situated at the Ca2+ stores, opens a plasma membrane Ca2+ pathway. This activity would be inhibited by Ca2+ inside the stores by a calmodulin-dependent mechanism.
This article has been cited by other articles:
|
|
 |
|
  Q. Ke, Y.-F. Xiao, J. A. Bradbury, J. P. Graves, L. M. DeGraff, J. M. Seubert, and D. C. Zeldin Electrophysiological Properties of Cardiomyocytes Isolated from CYP2J2 Transgenic Mice Mol. Pharmacol., October 1, 2007; 72(4): 1063 - 1073. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Chung, A. Limnander, T. Kurosaki, A. Weiss, and J. I. Korenbrot Coupling Ca2+ store release to Icrac channel activation in B lymphocytes requires the activity of Lyn and Syk kinases J. Cell Biol., April 23, 2007; 177(2): 317 - 328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-F. Xiao, Q. Ke, J. M. Seubert, J. A. Bradbury, J. Graves, L. M. DeGraff, J. R. Falck, K. Krausz, H. V. Gelboin, J. P. Morgan, et al. Enhancement of Cardiac L-Type Ca2+ Currents in Transgenic Mice with Cardiac-Specific Overexpression of CYP2J2 Mol. Pharmacol., December 1, 2004; 66(6): 1607 - 1616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Jambrina, R. Alonso, M. Alcalde, M. del Carmen Rodriguez, A. Serrano, C. Martinez-A., J. Garcia-Sancho, and M. Izquierdo Calcium Influx through Receptor-operated Channel Induces Mitochondria-triggered Paraptotic Cell Death J. Biol. Chem., April 11, 2003; 278(16): 14134 - 14145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lax, F. Soler, and F. Fernandez-Belda Inhibition of sarcoplasmic reticulum Ca2+-ATPase by miconazole Am J Physiol Cell Physiol, July 1, 2002; 283(1): C85 - C92. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wu, J. Sangerman, M. Li, G. H. Brough, S. R. Goodman, and T. Stevens Essential control of an endothelial cell ISOC by the spectrin membrane skeleton J. Cell Biol., September 17, 2001; 154(6): 1225 - 1234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rossato, A. Nogara, M. Merico, A. Ferlin, A. Garolla, and C. Foresta Store-Operated Calcium Influx and Stimulation of Steroidogenesis in Rat Leydig Cells: Role of Ca2+-Activated K+ Channels Endocrinology, September 1, 2001; 142(9): 3865 - 3872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Tiffert, H. Ginsburg, M. Krugliak, B. C. Elford, and V. L. Lew Potent antimalarial activity of clotrimazole in in vitro cultures of Plasmodium falciparum PNAS, January 4, 2000; 97(1): 331 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Ivey, A. H. Stephenson, and M. I. Townsley Involvement of cytochrome P-450 enzyme activity in the control of microvascular permeability in canine lung Am J Physiol Lung Cell Mol Physiol, October 1, 1998; 275(4): L756 - L763. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Adams, E. R. Meyer, and T. J. Cicero Imidazoles Suppress Rat Testosterone Secretion and Testicular Interstitial Fluid Formation In Vivo Biol Reprod, August 1, 1998; 59(2): 248 - 254. [Abstract] [Full Text]
|
|
|
|
|
|
A. M. Hofer, C. Fasolato, and T. Pozzan Capacitative Ca2+ Entry Is Closely Linked to the Filling State of Internal Ca2+ Stores: A Study Using Simultaneous Measurements of ICRAC and Intraluminal [Ca2+] J. Cell Biol., January 26, 1998; 140(2): 325 - 334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. W. Hopf, P. Reddy, J. Hong, and R. A. Steinhardt A Capacitative Calcium Current in Cultured Skeletal Muscle Cells Is Mediated by the Calcium-specific Leak Channel and Inhibited by Dihydropyridine Compounds J. Biol. Chem., September 13, 1996; 271(37): 22358 - 22367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Vostal and B. Shafer Thapsigargin-induced Calcium Influx in the Absence of Detectable Tyrosine Phosphorylation in Human Platelets J. Biol. Chem., August 9, 1996; 271(32): 19524 - 19529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. Toescu and O. H. Petersen Region-specific Activity of the Plasma Membrane Ca[IMAGE]Pump and Delayed Activation of Ca[IMAGE]Entry Characterize the Polarized, Agonist-evoked Ca[IMAGE]Signals in Exocrine Cells J. Biol. Chem., April 14, 1995; 270(15): 8528 - 8535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Thomas and M. R. Hanley Evaluation of Calcium Influx Factors from Stimulated Jurkat T-lymphocytes by Microinjection into Xenopus Oocytes J. Biol. Chem., March 24, 1995; 270(12): 6429 - 6432. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
