| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The FASEB Journal, Vol 5, 2085-2092, Copyright © 1991 by The Federation of American Societies for Experimental Biology
RESEARCH COMMUNICATIONS |
RD Curran, FK Ferrari, PH Kispert, J Stadler, DJ Stuehr, RL Simmons and TR Billiar
Department of Surgery, University of Pittsburgh, School of Medicine, Pennsylvania 15261.
Hepatocytes are stimulated to produce nitric oxide (NO.) from L- arginine in response to conditioned Kupffer cell medium or a combination of cytokines. Associated with the production of NO.in hepatocytes, there is a profound decrease in total protein synthesis ([3H]leucine incorporation). This report demonstrates that authentic NO.and the NO.-generating compound S-nitroso-N-acetylpenicillamine inhibit hepatocyte total protein synthesis in a reversible and concentration-dependent fashion. In parallel with the suppression of hepatocyte total protein synthesis, authentic NO.inhibits the production of two specific hepatocyte proteins, albumin and fibrinogen, without influencing the quantity of albumin mRNA. Although authentic NO.induces a rapid increase in cGMP levels in hepatocytes, the addition of the cGMP analog 8-bromoguanosine 3':5' cyclic monophosphate to unstimulated HC cultures does not reproduce the inhibition of total protein synthesis. These data show that NO.is the hepatocyte L-arginine metabolite that inhibits protein synthesis. Furthermore, these findings indicate that NO.does not inhibit hepatocyte protein synthesis solely through the activation of soluble guanylate cyclase but appears to affect a translational or posttranslational process.
This article has been cited by other articles:
|
|
 |
|
  S. C. Land and C. Rae iNOS initiates and sustains metabolic arrest in hypoxic lung adenocarcinoma cells: mechanism of cell survival in solid tumor core Am J Physiol Cell Physiol, October 1, 2005; 289(4): C918 - C933. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J Bruins, P. B Soeters, W. H Lamers, and N. E. Deutz L-Arginine supplementation in pigs decreases liver protein turnover and increases hindquarter protein turnover both during and after endotoxemia Am. J. Clinical Nutrition, June 1, 2002; 75(6): 1031 - 1044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Morin, S. M. Karr, R. A. Faris, and P. A. Gruppuso Developmental variability in expression and regulation of inducible nitric oxide synthase in rat intestine Am J Physiol Gastrointest Liver Physiol, August 1, 2001; 281(2): G552 - G559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. H. N. Hoebe, R. F. Witkamp, J. Fink-Gremmels, A. S. J. P. A. M. Van Miert, and M. Monshouwer Direct cell-to-cell contact between Kupffer cells and hepatocytes augments endotoxin-induced hepatic injury Am J Physiol Gastrointest Liver Physiol, April 1, 2001; 280(4): G720 - G728. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Pervin, R. Singh, and G. Chaudhuri Nitric oxide-induced cytostasis and cell cycle arrest of a human breast cancer cell line (MDA-MB-231): Potential role of cyclin D1 PNAS, March 13, 2001; 98(6): 3583 - 3588. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Zaragoza, C. J. Ocampo, M. Saura, C. Bao, M. Leppo, A. Lafond-Walker, D. R. Thiemann, R. Hruban, and C. J. Lowenstein Inducible Nitric Oxide Synthase Protection Against Coxsackievirus Pancreatitis J. Immunol., November 15, 1999; 163(10): 5497 - 5504. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhang, L. W. Velsor, J. M. Patel, E. M. Postlethwait, and E. R. Block Nitric oxide-induced reduction of lung cell and whole lung thioredoxin expression is regulated by NF-kappa B Am J Physiol Lung Cell Mol Physiol, October 1, 1999; 277(4): L787 - L793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang, Y. D. Li, J. M. Patel, and E. R. Block Thioredoxin overexpression prevents NO-induced reduction of NO synthase activity in lung endothelial cells Am J Physiol Lung Cell Mol Physiol, August 1, 1998; 275(2): L288 - L293. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. Sanders, E. S. Siekierski, J. D. Porter, S. M. Richards, and D. Proud Nitric Oxide Inhibits Rhinovirus-Induced Cytokine Production and Viral Replication in a Human Respiratory Epithelial Cell Line J. Virol., February 1, 1998; 72(2): 934 - 942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Horie, R. Wolf, and D. N. Granger Role of nitric oxide in gut ischemia-reperfusion-induced hepatic microvascular dysfunction Am J Physiol Gastrointest Liver Physiol, November 1, 1997; 273(5): G1007 - G1013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Stockert and Q. Ren Cytoplasmic Protein mRNA Interaction Mediates cGMP-modulated Translational Control of the Asialoglycoprotein Receptor J. Biol. Chem., April 4, 1997; 272(14): 9161 - 9165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tabuchi, E. Oh, A. Taoka, H. Sakurai, T. Tsuchiya, and M. Tsuda Rapid Attenuation of AP-1 Transcriptional Factors Associated with Nitric Oxide (NO)-mediated Neuronal Cell Death J. Biol. Chem., December 6, 1996; 271(49): 31061 - 31067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Kolpakov, D. Gordon, and T. J. Kulik Nitric Oxide–Generating Compounds Inhibit Total Protein and Collagen Synthesis in Cultured Vascular Smooth Muscle Cells Circ. Res., February 1, 1995; 76(2): 305 - 309. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
