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The FASEB Journal, Vol 4, 2528-2532, Copyright © 1990 by The Federation of American Societies for Experimental Biology

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Efficient production of chicken egg yolk antibodies against a conserved mammalian protein

M Gassmann, P Thommes, T Weiser and U Hubscher
Department of Pharmacology and Biochemistry, University of Zurich- Irchel, Switzerland.

The egg yolk of immunized chicken is a rich and inexpensive source of specific polyclonal antibodies. In this paper we show that 20-30 micrograms of a highly conserved mammalian protein, as exemplified by proliferating cell nuclear antigen, are sufficient to induce an immune response. Immunoblot analysis revealed that specific antibodies appeared 20 days after immunization, reached a plateau after 30 days, and remained high until at least day 81. A total amount of 4 g immunoglobulin was extracted from 62 eggs of one immunized hen, yielding approximately 130 mg of specific antibodies.


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Copyright © 1990 by The Federation of American Societies for Experimental Biology.