FASEB J. Avanti Polar Lipids
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Order Full text via Infotrieve
Right arrow Buy
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Minakami, H.
Right arrow Articles by Fridovich, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Minakami, H.
Right arrow Articles by Fridovich, I.

The FASEB Journal, Vol 4, 3239-3244, Copyright © 1990 by The Federation of American Societies for Experimental Biology

RESEARCH COMMUNICATIONS

Relationship between growth of Escherichia coli and susceptibility to the lethal effect of paraquat

H Minakami and I Fridovich
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.

Paraquat, which mediates increased O2- production within Escherichia coli, inhibits growth without causing cell death in a minimal medium, whereas it allows growth while decreasing viability in a rich medium. Agents or conditions that inhibit growth in the paraquat-containing rich medium prevent this loss of viability. Antibiotics, chelating agents, nutritional paucity, and excess paraquat all acted this way. NaCl added to 0.2 M to the plates used for enumeration after paraquat exposure did not significantly decrease viable cell counts, although it did so after exposure to a cationic detergent. DNA, rather than cell membranes, thus seems to be a more likely target for the oxygen-derived free radicals engendered by the cycles of enzymatic reduction and autoxidation of paraquat. Inhibition of growth decreases the likelihood that DNA replication will precede DNA repair. Investigators seeking to evaluate the bactericidal actions of paraquat under a variety of conditions will need to be cognizant of this need for growth.


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
A. R. Krapp, R. E. Rodriguez, H. O. Poli, D. H. Paladini, J. F. Palatnik, and N. Carrillo
The Flavoenzyme Ferredoxin (Flavodoxin)-NADP(H) Reductase Modulates NADP(H) Homeostasis during the soxRS Response of Escherichia coli
J. Bacteriol., March 1, 2002; 184(5): 1474 - 1480.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1990 by The Federation of American Societies for Experimental Biology.