| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The FASEB Journal, Vol 3, 1850-1854, Copyright © 1989 by The Federation of American Societies for Experimental Biology
RESEARCH COMMUNICATIONS |
M Nakahiro, JZ Yeh, E Brunner and T Narahashi
Department of Pharmacology, Northwestern University Medical School, Chicago, Illinois 60611.
The effects of halothane, isoflurane, and enflurane on ionic currents induced by bath application of gamma-amino-butyric acid (GABA) were studied with the rat dorsal root ganglion neurons maintained in primary culture. The whole-cell patch clamp technique was used to record the current. In normal neurons before exposure to anesthetics, GABA at low concentrations (1-3 x 10(-6) M) induced a small sustained inward current. At higher concentrations (3 x 10(-5) M-1 x 10(-3) M), GABA induced a large inward current, which decayed to a steady-state level (desensitization). Halothane (0.86 mM), isoflurane (0.96 mM), and enflurane (1.89 mM), each equivalent to the respective 2 minimum alveolar concentration (MAC) units, augmented the sustained current evoked by 3 x 10(-6) M GABA to 330-350% of control and the peak current evoked by 3 x 10(-5) M of GABA to 136-145% of control. The decay phase of the current was accelerated by the anesthetics, the time for the current to decline to 70% of the peak being reduced to 23-39% of control. In contrast, the densitized steady-state current evoked by high concentrations of GABA was decreased by anesthetics. In conclusion, general anesthetics exert a dual effect on the GABA receptor channel complex: to potentiate the nondesensitized (both peak and sustained) current and to suppress the desensitized steady-state current. The potentiation of the GABA receptor channel response may be a primary action of anesthetics leading to surgical anesthesia.
This article has been cited by other articles:
|
|
 |
|
  M. Perouansky, M. I. Banks, and R. A. Pearce The Differential Effects of the Nonimmobilizer 1,2-Dichlorohexafluorocyclobutane (F6, 2N) and Isoflurane on Extrasynaptic Gamma-Aminobutyric AcidA Receptors Anesth. Analg., June 1, 2005; 100(6): 1667 - 1673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kitamura, R. Sato, W. Marszalec, J. Z. Yeh, R. Ogawa, and T. Narahashi Halothane and Propofol Modulation of {gamma}-Aminobutyric AcidA Receptor Single-Channel Currents Anesth. Analg., August 1, 2004; 99(2): 409 - 415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Hall, K. C. Rowan, R. J. N. Stevens, J. C. Kelley, and N. L. Harrison The Effects of Isoflurane on Desensitized Wild-Type and {alpha}1(S270H) {gamma}-Aminobutyric Acid Type A Receptors Anesth. Analg., May 1, 2004; 98(5): 1297 - 1304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ochiai, T. Yamada, S. Kiyama, T. Nakaoji, and J. Takeda Bispectral Index as an Indicator of Seizure Inducibility in Electroconvulsive Therapy Under Thiopental Anesthesia Anesth. Analg., April 1, 2004; 98(4): 1030 - 1035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kitamura, W. Marszalec, J. Z. Yeh, and T. Narahashi Effects of Halothane and Propofol on Excitatory and Inhibitory Synaptic Transmission in Rat Cortical Neurons J. Pharmacol. Exp. Ther., January 1, 2003; 304(1): 162 - 171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Raines, R. J. Claycomb, and S. A. Forman Modulation of GABAA Receptor Function by Nonhalogenated Alkane Anesthetics: The Effects on Agonist Enhancement, Direct Activation, and Inhibition Anesth. Analg., January 1, 2003; 96(1): 112 - 118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Eckenhoff PROMISCUOUS LIGANDS AND ATTRACTIVE CAVITIES: How do the inhaled anesthetics work? Mol. Interv., December 1, 2001; 1(5): 258 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Mori, X. Zhao, Y. Zuo, G. L. Aistrup, K. Nishikawa, W. Marszalec, J. Z. Yeh, and T. Narahashi Modulation of Neuronal Nicotinic Acetylcholine Receptors by Halothane in Rat Cortical Neurons Mol. Pharmacol., April 1, 2001; 59(4): 732 - 743. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Nishikawa and M. B. MacIver Membrane and Synaptic Actions of Halothane on Rat Hippocampal Pyramidal Neurons and Inhibitory Interneurons J. Neurosci., August 15, 2000; 20(16): 5915 - 5923. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Narahashi Neuroreceptors and Ion Channels as the Basis for Drug Action: Past, Present, and Future J. Pharmacol. Exp. Ther., July 1, 2000; 294(1): 1 - 26. [Abstract] [Full Text]
|
|
|
|
|
|
S. Neumahr, G. Hapfelmeier, M. Scheller, H. Schneck, C. Franke, and E. Kochs Dual Action of Isoflurane on the {gamma}-aminobutyric Acid (GABA)-Mediated Currents Through Recombinant {alpha}1{beta}2{gamma}2L-GABAA-Receptor Channels Anesth. Analg., May 1, 2000; 90(5): 1184 - 1190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hamakawa, Z.-P. Feng, N. Grigoriv, T. Inoue, M. Takasaki, S. Roth, K. Lukowiak, S. U. Hasan, and N. I. Syed Sevoflurane Induced Suppression of Inhibitory Synaptic Transmission Between Soma-Soma Paired Lymnaea Neurons J Neurophysiol, November 1, 1999; 82(5): 2812 - 2819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kurata, W. Marszalec, J. Z. Yeh, and T. Narahashi Agonist and Potentiation Actions of n-Octanol on gamma -Aminobutyric Acid Type A Receptors Mol. Pharmacol., June 1, 1999; 55(6): 1011 - 1019. [Abstract] [Full Text]
|
|
|
|
|
|
E. I Eger II, D. D. Koblin, J. Sonner, D. Gong, M. J. Laster, P. Ionescu, M. J. Halsey, and T. Hudlicky Nonimmobilizers and Transitional Compounds May Produce Convulsions by Two Mechanisms Anesth. Analg., April 1, 1999; 88(4): 884 - 884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Wakasugi, K. Hirota, S. H. Roth, and Y. Ito The Effects of General Anesthetics on Excitatory and Inhibitory Synaptic Transmission in Area CA1 of the Rat Hippocampus In Vitro Anesth. Analg., March 1, 1999; 88(3): 676 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Krasowski, S. E. Finn, Q. Ye, and N. L. Harrison Trichloroethanol Modulation of Recombinant GABAA, Glycine and GABA rho 1 Receptors J. Pharmacol. Exp. Ther., March 1, 1998; 284(3): 934 - 942. [Abstract] [Full Text]
|
|
|
|
|
|
M. D. Krasowski, V. V. Koltchine, C. E. Rick, Q. Ye, S. E. Finn, and N. L. Harrison Propofol and Other Intravenous Anesthetics Have Sites of Action on the gamma -Aminobutyric Acid Type A Receptor Distinct from That for Isoflurane Mol. Pharmacol., March 1, 1998; 53(3): 530 - 538. [Abstract] [Full Text]
|
|
|
|
|
|
B. Antkowiak and D. Heck Effects of the Volatile Anesthetic Enflurane on Spontaneous Discharge Rate and GABAA-Mediated Inhibition of Purkinje Cells in Rat Cerebellar Slices J Neurophysiol, May 1, 1997; 77(5): 2525 - 2538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Jenkins, E. P. Greenblatt, H. J. Faulkner, E. Bertaccini, A. Light, A. Lin, A. Andreasen, A. Viner, J. R. Trudell, and N. L. Harrison Evidence for a Common Binding Cavity for Three General Anesthetics within the GABAA Receptor J. Neurosci., March 15, 2001; 21(6): RC136 - RC136. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
