FASEB J. Cell Migration Consortium
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McConkey, D. J.
Right arrow Articles by Orrenius, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McConkey, D. J.
Right arrow Articles by Orrenius, S.

The FASEB Journal, Vol 3, 1843-1849, Copyright © 1989 by The Federation of American Societies for Experimental Biology

RESEARCH COMMUNICATIONS

Calcium-activated DNA fragmentation kills immature thymocytes

DJ McConkey, P Hartzell, P Nicotera and S Orrenius
Department of Toxicology, Karolinska Institutet, Stockholm, Sweden.

Glucocorticoid hormones kill immature thymocytes by activating a self- destructive process that involves extensive DNA fragmentation. It has been demonstrated that thymocyte suicide is dependent on an early, sustained increase in cytosolic Ca2+ concentration, and new protein synthesis, but the biochemical lesion that leads to cell death has not been established. To determine whether endonuclease activation or activation of another Ca2+-dependent process could mediate cell killing, we treated thymocytes with the glucocorticoid methylprednisolone in the presence of inhibitors of various Ca2+- dependent degradative enzymes. The role of poly(ADP-ribose) polymerase, an enzyme known to be activated by DNA damage, was also assessed. Glucocorticoid-induced chromatin cleavage and cell killing were blocked by the endonuclease inhibitor aurintricarboxylic acid, whereas inhibitors of other Ca2+-dependent degradative processes or of poly(ADP- ribose) polymerase did not abrogate cell death. In addition, stimulation of thymocyte DNA fragmentation by the Ca2+ ionophore A23187 resulted in cell killing that could be blocked by the endonuclease inhibitor. Together, our results suggest that thymocyte suicide is caused by extensive Ca2+-stimulated DNA fragmentation.


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
D. W.-C. Li, J.-P. Liu, Y.-W. Mao, H. Xiang, J. Wang, W.-Y. Ma, Z. Dong, H. M. Pike, R. E. Brown, and J. C. Reed
Calcium-activated RAF/MEK/ERK Signaling Pathway Mediates p53-dependent Apoptosis and Is Abrogated by {alpha}B-Crystallin through Inhibition of RAS Activation
Mol. Biol. Cell, September 1, 2005; 16(9): 4437 - 4453.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
C. Vindis, M. Elbaz, I. Escargueil-Blanc, N. Auge, A. Heniquez, J.-C. Thiers, A. Negre-Salvayre, and R. Salvayre
Two Distinct Calcium-Dependent Mitochondrial Pathways Are Involved in Oxidized LDL-Induced Apoptosis
Arterioscler. Thromb. Vasc. Biol., March 1, 2005; 25(3): 639 - 645.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Haimsohn, R. Beery, A. Karasik, H. Kanety, and A. Geier
Aurintricarboxylic Acid Induces a Distinct Activation of the IGF-I Receptor Signaling within MDA-231 Cells
Endocrinology, March 1, 2002; 143(3): 837 - 845.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. Beery, M. Haimsohn, N. Wertheim, R. Hemi, U. Nir, A. Karasik, H. Kanety, and A. Geier
Activation of the Insulin-Like Growth Factor 1 Signaling Pathway by the Antiapoptotic Agents Aurintricarboxylic Acid and Evans Blue
Endocrinology, July 1, 2001; 142(7): 3098 - 3107.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Fettucciari, E. Rosati, L. Scaringi, P. Cornacchione, G. Migliorati, R. Sabatini, I. Fetriconi, R. Rossi, and P. Marconi
Group B Streptococcus Induces Apoptosis in Macrophages
J. Immunol., October 1, 2000; 165(7): 3923 - 3933.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
O. VIEIRA, I. ESCARGUEIL-BLANC, G. JÜRGENS, C. BORNER, L. ALMEIDA, R. SALVAYRE, and A. NÈGRE-SALVAYRE
Oxidized LDLs alter the activity of the ubiquitin-proteasome pathway: potential role in oxidized LDL-induced apoptosis
FASEB J, March 1, 2000; 14(3): 532 - 542.
[Abstract] [Full Text]

Home page
 J. Nutr.Home page
W. G. Kirlin, J. Cai, M. J. DeLong, E. J. Patten, and D. P. Jones
Dietary Compounds That Induce Cancer Preventive Phase 2 Enzymes Activate Apoptosis at Comparable Doses in HT29 Colon Carcinoma Cells
J. Nutr., October 1, 1999; 129(10): 1827 - 1835.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
P. W. Mesner Jr., K. C. Bible, L. M. Martins, T. J. Kottke, S. M. Srinivasula, P. A. Svingen, T. J. Chilcote, G. S. Basi, J. S. Tung, S. Krajewski, et al.
Characterization of Caspase Processing and Activation in HL-60 Cell Cytosol Under Cell-free Conditions. NUCLEOTIDE REQUIREMENT AND INHIBITOR PROFILE
J. Biol. Chem., August 6, 1999; 274(32): 22635 - 22645.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
R. Sacedon, A. Vicente, A. Varas, E. Jimenez, J. J. Munoz, and A. G. Zapata
Glucocorticoid-mediated regulation of thymic dendritic cell function
Int. Immunol., August 1, 1999; 11(8): 1217 - 1224.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
J. Alcouffe, S. Caspar-Bauguil, V. Garcia, R. Salvayre, M. Thomsen, and H. Benoist
Oxidized low density lipoproteins induce apoptosis in PHA-activated peripheral blood mononuclear cells and in the Jurkat T-cell line
J. Lipid Res., July 1, 1999; 40(7): 1200 - 1210.
[Abstract] [Full Text]

Home page
 FASEB J.Home page
O. MEILHAC, I. ESCARGUEIL-BLANC, J.-C. THIERS, R. SALVAYRE, and A. NÈGRE-SALVAYRE
Bcl-2 alters the balance between apoptosis and necrosis, but does not prevent cell death induced by oxidized low density lipoproteins
FASEB J, March 1, 1999; 13(3): 485 - 494.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
T. Kasai, K. Ohguchi, S. Nakashima, Y. Ito, T. Naganawa, N. Kondo, and Y. Nozawa
Increased Activity of Oleate-Dependent Type Phospholipase D During Actinomycin D-Induced Apoptosis in Jurkat T Cells
J. Immunol., December 15, 1998; 161(12): 6469 - 6474.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Escargueil-Blanc, N. Andrieu-Abadie, S. Caspar-Bauguil, R. Brossmer, T. Levade, A. Negre-Salvayre, and R. Salvayre
Apoptosis and Activation of the Sphingomyelin-Ceramide Pathway Induced by Oxidized Low Density Lipoproteins Are Not Causally Related in ECV-304 Endothelial Cells
J. Biol. Chem., October 16, 1998; 273(42): 27389 - 27395.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C.-Y. Chen, J. Liou, L. W. Forman, and D. V. Faller
Differential Regulation of Discrete Apoptotic Pathways by Ras
J. Biol. Chem., July 3, 1998; 273(27): 16700 - 16709.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. C. Schittny, V. Djonov, A. Fine, and P. H. Burri
Programmed Cell Death Contributes to Postnatal Lung Development
Am. J. Respir. Cell Mol. Biol., June 1, 1998; 18(6): 786 - 793.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
B. Dallaporta, T. Hirsch, S. A. Susin, N. Zamzami, N. Larochette, C. Brenner, I. Marzo, and G. Kroemer
Potassium Leakage During the Apoptotic Degradation Phase
J. Immunol., June 1, 1998; 160(11): 5605 - 5615.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
I. Escargueil-Blanc, O. Meilhac, M.-T. Pieraggi, J.-F. Arnal, R. Salvayre, and A. Negre-Salvayre
Oxidized LDLs Induce Massive Apoptosis of Cultured Human Endothelial Cells Through a Calcium-Dependent Pathway: Prevention by Aurintricarboxylic Acid
Arterioscler. Thromb. Vasc. Biol., February 1, 1997; 17(2): 331 - 339.
[Abstract] [Full Text]

Home page
 Stem CellsHome page
D. McConkey and S Orrenius
Signal transduction pathways in apoptosis
Stem Cells, November 1, 1996; 14(6): 619 - 631.
[Abstract]

Home page
 NEJMHome page
J. Narula, N. Haider, R. Virmani, T. G. DiSalvo, F. D. Kolodgie, R. J. Hajjar, U. Schmidt, M. J. Semigran, G. W. Dec, and B.-A. Khaw
Apoptosis in Myocytes in End-Stage Heart Failure
N. Engl. J. Med., October 17, 1996; 335(16): 1182 - 1189.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
DavidJ. McConkey and D. J. McConkey
Calcium-dependent, Interleukin 1beta -converting Enzyme Inhibitor-insensitive Degradation of Lamin B1 and DNA Fragmentation in Isolated Thymocyte Nuclei
J. Biol. Chem., September 13, 1996; 271(37): 22398 - 22406.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Fraser, S. Tynan, A Papaioannou, C. Ireland, and S. Pittman
Endo-exonuclease of human leukaemic cells: evidence for a role in apoptosis
J. Cell Sci., January 9, 1996; 109(9): 2343 - 2360.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
A. Torriglia, E. Chaudun, F.ço. Chany-Fournier, J.-C. Jeanny, Y. Courtois, and M.-F. Counis
Involvement of DNase II in Nuclear Degeneration during Lens Cell Differentiation
J. Biol. Chem., December 1, 1995; 270(48): 28579 - 28585.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. A. Lagarkova, O. V. Iarovaia, and S. V. Razin
Large-scale Fragmentation of Mammalian DNA in the Course of Apoptosis Proceeds via Excision of Chromosomal DNA Loops and Their Oligomers
J. Biol. Chem., September 1, 1995; 270(35): 20239 - 20241.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Pérez-Sala, D. Collado-Escobar, and F. Mollinedo
Intracellular Alkalinization Suppresses Lovastatin-induced Apoptosis in HL-60 Cells through the Inactivation of a pH-dependent Endonuclease
J. Biol. Chem., March 17, 1995; 270(11): 6235 - 6242.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Li and A. Eastman
Apoptosis in an Interleukin-2-dependent Cytotoxic T Lymphocyte Cell Line Is Associated with Intracellular Acidification
J. Biol. Chem., February 17, 1995; 270(7): 3203 - 3211.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
B. Gjertsen, L. Cressey, S Ruchaud, G Houge, M Lanotte, and S. Doskeland
Multiple apoptotic death types triggered through activation of separate pathways by cAMP and inhibitors of protein phosphatases in one (IPC leukemia) cell line
J. Cell Sci., January 12, 1994; 107(12): 3363 - 3377.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
G. Kulkarni and C. McCulloch
Serum deprivation induces apoptotic cell death in a subset of Balb/c 3T3 fibroblasts
J. Cell Sci., January 5, 1994; 107(5): 1169 - 1179.
[Abstract] [PDF]

Home page
 ScienceHome page
L Juntti-Berggren, O Larsson, P Rorsman, C Ammala, K Bokvist, K Wahlander, P Nicotera, J Dypbukt, S Orrenius, A Hallberg, et al.
Increased activity of L-type Ca2+ channels exposed to serum from patients with type I diabetes
Science, July 2, 1993; 261(5117): 86 - 90.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
F Oberhammer, G Fritsch, M Schmied, M Pavelka, D Printz, T Purchio, H Lassmann, and R Schulte-Hermann
Condensation of the chromatin at the membrane of an apoptotic nucleus is not associated with activation of an endonuclease
J. Cell Sci., January 2, 1993; 104(2): 317 - 326.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
A. B. Scoltock, C. D. Bortner, G. St. J. Bird, J. W. Putney Jr., and J. A. Cidlowski
A Selective Requirement for Elevated Calcium in DNA Degradation, but Not Early Events in Anti-Fas-induced Apoptosis
J. Biol. Chem., September 22, 2000; 275(39): 30586 - 30596.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1989 by The Federation of American Societies for Experimental Biology.