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The FASEB Journal, Vol 3, 174-177, Copyright © 1989 by The Federation of American Societies for Experimental Biology

RESEARCH COMMUNICATIONS

Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration [published erratum appears in FASEB J 1989 May;3(7):1873]

JC Dunn, ML Yarmush, HG Koebe and RG Tompkins
Department of Surgery, Massachusetts General Hospital, Boston 02114.

Adult rat hepatocytes cultured in a collagen sandwich system maintained normal morphology and a physiological rate of albumin secretion for at least 42 days. Hepatocytes cultured on a single layer of collagen gel essentially ceased albumin secretion within 1 wk but could recover function with the overlay of a second layer of collagen gel. This culture configuration more closely mimics the hepatocytes' in vivo environment and provides a simple method for their long-term maintenance.


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Copyright © 1989 by The Federation of American Societies for Experimental Biology.