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The FASEB Journal, Vol 3, 37-43, Copyright © 1989 by The Federation of American Societies for Experimental Biology

RESEARCH COMMUNICATIONS

Nuclear disintegration of target cells by killer B lymphocytes from tumor-bearing mice

DM Lopez, BB Blomberg, RR Padmanabhan and LY Bourguignon
Department of Microbiology, University of Miami School of Medicine, Florida 33101.

Normal murine B lymphocytes are not known to be effectors of the Fc receptor-mediated, antibody-dependent cellular cytotoxicity (ADCC). In contrast, we report here that highly purified splenic B cells from mammary tumor-bearing mice develop the potential of lysing antibody- coated target cells. These lymphocytes are characterized by being G-10 nonadherent, nylon wool adherent, sIg+, FcR+, Thy 1.2-, asialo GM1-, and the immunoglobulin heavy-chain genes of both chromosomes are rearranged. The lytic reaction is characterized by a noninterdigitating binding and by the appearance of endocytotic vesicles in the target cells. Nuclear disintegration occurs 18 h after initial effector-target cell conjugate formation. At such time, only minor cytoplasmic membrane alterations are evident. The emergence of killer B cells in tumor- bearing hosts indicates that all lymphoreticular cell types bearing Fc receptors are capable of mediating ADCC.




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Copyright © 1989 by The Federation of American Societies for Experimental Biology.