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Circadian clock and output genes are rhythmically expressed in extratesticular ducts and accessory organs of mice

Piotr Bebas^*,, Cheri P. Goodall, Magda Majewska, Adam Neumann^*, Jadwiga M. Giebultowicz and Patrick E. Chappell^,1

^* Department of Animal Physiology, Faculty of Biology, University of Warsaw, Warsaw, Poland; and

Department of Zoology, Oregon State University, Corvallis, Oregon, USA

¹Correspondence: Department of Zoology, Oregon State University, Cordley Hall 3029, Corvallis, OR 97331-2914, USA. E-mail: patrick.chappell{at}science.oregonstate.edu

Circadian clocks regulate multiple rhythms in mammalian tissues. In most organs core clock gene expression is oscillatory, with negative components Per and Cry peaking in antiphase to Bmal1. A notable exception is the testis, where clock genes seem nonrhythmic. Earlier mammalian studies, however, did not examine clock expression patterns in accessory ductal tissue required for sperm maturation and transport. Previous studies in insects demonstrated control of sperm maturation in vas deferens by a local circadian system. Sperm ducts express clock genes and display circadian pH changes controlled by vacuolar-type H⁺-ATPase and carbonic anhydrase (CA-II). It is unknown whether sperm-processing rhythms are conserved beyond insects. To address this question in mice housed in a light-dark environment, we examined temporal patterns of mPer1 and Bmal1 gene expression and protein abundance in epididymis, vas deferens, seminal vesicles, and prostate. Results demonstrate variable tissue-specific patterns of expression of the two genes, with variations in levels of clock proteins and their nucleo-cytoplasmic cycling observed among examined tissues. Strikingly, mPer1 and Bmal1 mRNA and proteins oscillate in antiphase in the prostate, with similar peak-trough patterns as observed in the suprachiasmatic nuclei, the brain’s central clock. Genes encoding CA and a V-ATPase subunit, which are rhythmically expressed in sperm ducts of moths, are also rhythmic in some segments of murine sperm ducts. Our data suggest that some sperm duct segments may contain peripheral circadian systems whereas others may express clock genes in a pleiotropic manner.—Bebas, P., Goodall, C. P., Majewska, M., Neumann, A., Giebultowicz, J. M., Chappell, P. E. Circadian clock and output genes are rhythmically expressed in extratesticular ducts and accessory organs of mice.

Key Words: V-ATPase • carbonic anhydrase • prostate • sperm maturation • testis

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