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A protein complex in the brush-border membrane explains a Hartnup disorder allele

Sonja Kowalczuk^*, Angelika Bröer^*, Nadine Tietze^*, Jessica M. Vanslambrouck, John E. J. Rasko^, and Stefan Bröer^*,1

^* School of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia;

Gene and Stem Cell Therapy Program, Centenary Institute of Cancer Medicine and Cell Biology, University of Sydney, Camperdown, Australia; and

Cell and Molecular Therapies, Sydney Cancer Centre, Royal Prince Alfred Hospital, Camperdown, Australia

¹Correspondence: School of Biochemistry and Molecular Biology, Australian National University, Linnaeus Way 41, Canberra, ACT 0200, Australia. E-mail: stefan.broeer{at}anu.edu.au

Protein absorption in the intestine is mediated by proteases and brush-border peptidases together with peptide and amino acid transporters. Neutral amino acids are generated by a variety of aminopeptidases and carboxypeptidases and are subsequently taken up by the amino acid transporter B⁰AT1 (SLC6A19), which is mutated in Hartnup disorder. Coexpression of B⁰AT1 together with the brush-border carboxypeptidase angiotensin-converting enzyme 2 (ACE2) in Xenopus laevis oocytes led to a dramatic increase of transporter expression at the oocyte surface. Other members of the SLC6 family were not stimulated by coexpression with ACE2. Addition of a peptide containing a carboxyterminal leucine residue to ACE2- and B⁰AT1-coexpressing oocytes caused inward currents due to Na⁺-leucine cotransport, demonstrating the formation of a metabolic complex. Coexpression of the Hartnup disorder causing mutation B⁰AT1(R240Q) showed reduced interaction with ACE2 and its renal paralogue collectrin. This would result in reduced surface expression in both kidney and intestine, thereby explaining the onset of the disorder in individuals carrying this mutation.—Kowalczuk, S., Bröer, A., Tietze, N., Vanslambrouck, J. M., Rasko, J. E. J., Bröer, S. A protein complex in the brush-border membrane explains a Hartnup disorder allele.

Key Words: amino acid transport • angiotensin-converting enzyme 2 • epithelial transport