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This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: fj.07-101485v1 22/7/2435    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Shin, S. S. Articles by Han, J. Y. PubMed PubMed Citation Articles by Shin, S. S. Articles by Han, J. Y.

Generation of transgenic quail through germ cell-mediated germline transmission

Sang Su Shin^*,1, Tae Min Kim^*,1, Sun Young Kim^*, Tae Wan Kim^*, Hee Won Seo^*, Seul Ki Lee, Se Chang Kwon, Gwan Sun Lee, Heebal Kim^*, Jeong Mook Lim^* and Jae Yong Han^*,2

^* Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea;

Avicore Biotechnology Institute, Incorporated, Gyeonggi-Do, Korea; and

Hanmi Research Center, Hanmi Pharm Company, Limited, Gyeonggi-Do, Korea

²Correspondence: Department of Agricultural Biotechnology, Seoul National University, Seoul, 151–921, Korea. E-mail: jaehan{at}snu.ac.kr

Here, we describe the production of transgenic quail via a germline transmission system using postmigratory gonadal primordial germ cells (gPGCs). gPGCs retrieved from the embryonic gonads of 5-day-old birds were transduced with a lentiviral vector and subsequently transferred into recipient embryos. Testcross and genetic analyses revealed that among three germline chimeric G0 quail, one male produced transgenic offspring; of 310 hatchlings from the transgenic germline chimera, 24 were identified as donor-derived offspring, and 6 were transgenic (6/310, 1.9%). Conventional transgenesis using stage X blastodermal embryos was also conducted, but the efficiency of transgenesis was similar between the two systems (<1.6 vs. 1.9% for the conventional and gPGC-mediated systems, respectively). However, substantial advantages can be gained from gPGC-mediated method in that it enables an induced germline modification, whereas direct retroviral transfer to stage X embryos causes mosaic integration. The use of gonadal PGCs for transgenesis may lead to the production of bioreactors.—Shin, S. S., Kim, T. M., Kim, S. Y., Kim, T. W., Seo, H. W., Lee, S. K., Kwon, S. C., Lee, G. S., Kim, H., Lim, J. M., Han, J. Y. Generation of transgenic quail through germ cell-mediated germline transmission.

Key Words: primordial germ cells • lentiviral vector • avian transgenesis