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Exercise-induced promotion of hippocampal cell proliferation requires β-endorphin

M. Koehl^*,1,2, P. Meerlo^,1, D. Gonzales^*, A. Rontal, F. W. Turek and D. N. Abrous^*

^* INSERM, U862 and University of Bordeaux, Bordeaux, France;

Department of Molecular Neurobiology, University of Groningen, Haren, The Netherlands; and

Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois, USA

²Correspondence: Centre de Recherche INSERM U862, Physiopathologie de la Plasticité Neuronale, 146 Rue Léo Saignat, 33077 Bordeaux Cedex, France. E-mail: koehl{at}bordeaux.inserm.fr

Adult hippocampal neurogenesis is influenced by a variety of stimuli, including exercise, but the mechanisms by which running affects neurogenesis are not yet fully understood. Because β-endorphin, which is released in response to exercise, increases cell proliferation in vitro, we hypothesized that it could exert a similar effect in vivo and mediate the stimulatory effects of running on neurogenesis. We thus analyzed the effects of voluntary wheel-running on adult neurogenesis (proliferation, differentiation, survival/death) in wild-type and β-endorphin-deficient mice. In wild-type mice, exercise promoted cell proliferation evaluated by sacrificing animals 24 h after the last 5-bromo-2'-deoxyuridine (BrdU) pulse and by using endogenous cell cycle markers (Ki67 and pH₃). This was accompanied by an increased survival of 4-wk-old BrdU-labeled cells, leading to a net increase of neurogenesis. β-Endorphin deficiency had no effect in sedentary mice, but it completely blocked the running-induced increase in cell proliferation; this blockade was accompanied by an increased survival of 4-wk-old cells and a decreased cell death. Altogether, adult neurogenesis was increased in response to exercise in knockout mice. We conclude that β-endorphin released during running is a key factor for exercise-induced cell proliferation and that a homeostatic balance may regulate the final number of new neurons. Koehl, M., Meerlo, P., Gonzales, D., Rontal, A., Turek, F. W., Abrous, D. N. Exercise-induced promotion of hippocampal cell proliferation requires β-endorphin.

Key Words: running • opioids • cell survival • adult neurogenesis • homeostatic balance