HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: fj.07-104687v1 22/7/2223    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Wakao, H. Articles by Fujita, H. PubMed PubMed Citation Articles by Wakao, H. Articles by Fujita, H.

In vitro induction of natural killer T cells from embryonic stem cells prepared using somatic cell nuclear transfer

Hiroshi Wakao^*,,1, Rika Wakao^,2, Sakura Sakata, Kazuya Iwabuchi, Atsushi Oda^* and Hiroyoshi Fujita^*

^* Department of Environmental Biology, School of Medicine, and

Division of Immunobiology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan; and

Developmental Genetics Group and

Immune Regulation Group, Rikagaku Kenkyusho (RIKEN), Research Center for Allergy and Immunology, Yokohama, Japan

¹Correspondence: Department of Environmental Biology, School of Medicine, Hokkaido University, N15W7, Sapporo 060-8638, Japan. E-mail: hwakao{at}med.hokudai.ac.jp

The ectopic expression of the Notch receptor ligand delta-like 1 on stromal cells allows the induction of T cells from embryonic stem cells (ESCs). However, these in vitro-generated T cells are not transplantable because they are too immature to mount an immune response in an immunocompromised animal. We efficiently generated a subset of T cells called invariant natural killer T (iNKT) cells from ESCs derived from peripheral iNKT cells using somatic cell nuclear transfer (ntESCs). These iNKT cells matured autonomously in vivo and exhibited an adjuvant effect accompanying the production of interferon- in an antigen-specific manner. This adjuvant effect culminated in the inhibition of inoculated tumor cell growth. Our results indicate that ntESC-derived iNKT cells are transplantable lymphocytes that will be beneficial for the induction of immune tolerance and the treatment of autoimmune diseases, tumors, and infections.—Wakao, H., Wakao, R., Sakata, S., Iwabuchi, K., Oda, A., Fujita, H. In vitro induction of natural killer T cells from embryonic stem cells prepared using somatic cell nuclear transfer.

Key Words: adjuvant effect • in vitro differentiation • transplantable lymphocytes • tumor rejection