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β-Arrestins specifically constrain β2-adrenergic receptor signaling and function in airway smooth muscle

Deepak A. Deshpande^*, Barbara S. Theriot, Raymond B. Penn^*,1 and Julia K. L. Walker^,1

^* Department of Internal Medicine and Center for Human Genomics, Wake Forest University Health Sciences, Winston-Salem, North Carolina, USA; and

Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA

¹Correspondence: R.B.P., Medical Center Blvd., Center for Human Genomics, WFUHSC, Winston-Salem, NC, 27157. E-mail: rpenn{at}wfubmc.edu; J.K.L.W., Box 2641, Division of Pulmonary Medicine, DUMC, Durham, NC, 27710, USA. E-mail: walke082{at}mc.duke.edu

Chronic use of inhaled beta-agonists by asthmatics is associated with a loss of bronchoprotective effect and deterioration of asthma control. Beta-agonist-promoted desensitization of airway smooth muscle beta-2-adrenergic receptors, mediated by G protein-coupled receptor kinases and arrestins, is presumed to underlie these effects, but such a mechanism has never been demonstrated. Using in vitro, ex vivo, and in vivo murine models, we demonstrate that beta-arrestin-2 gene ablation augments beta-agonist-mediated airway smooth muscle relaxation, while augmenting beta-agonist-stimulated cyclic adenosine monophosphate production. In cultures of human airway smooth muscle, small interfering RNA-mediated knockdown of arrestins also augments beta-agonist-stimulated cyclic adenosine monophosphate production. Interestingly, signaling and function mediated by m2/m3 muscarinic acetylcholine receptors or prostaglandin E₂ receptors were not affected by either beta-arrestin-2 knockout or arrestin knockdown. Thus, arrestins are selective regulators of beta-2-adrenergic receptor signaling and function in airway smooth muscle. These results and our previous findings, which demonstrate a role for arrestins in the development of allergic inflammation in the lung, identify arrestins as potentially important therapeutic targets for obstructive airway diseases.—Deshpande, D. A., Theriot, B. S., Penn, R. B., Walker, J. K. L. β-Arrestins specifically constrain β2-adrenergic receptor signaling and function in airway smooth muscle.