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Glucocorticoid suppression of CX₃CL1 (fractalkine) by reduced gene promoter recruitment of NF-B

Experimental Studies, Airway Disease Section, National Heart and Lung Institute, Imperial College London, UK

¹Correspondence: Airway Disease, National Heart and Lung Institute, Guy Scadding Bldg., Imperial College London, Dovehouse St., London, SW3 6LY, UK. E-mail: p.bhavsar{at}imperial.ac.uk

Glucocorticoids are an important anti-inflammatory treatment of many inflammatory diseases including asthma. However, the mechanisms by which they mediate their suppressive effects are not fully understood. Respiratory epithelial cells are a source of CX₃CL1 (fractalkine), which mediates cell adhesion and acts as a chemoattractant for monocytes, T cells, and mast cells. We show, in lung A549 epithelial cells, that the tumor necrosis factor- (TNF-) and IFN synergistically induced protein release and mRNA expression of CX₃CL1 is inhibited by dexamethasone, without interfering with cytokine-induced nuclear translocation of NF-B, and by an inhibitor of IB kinase 2, AS602868. DNA binding assays confirmed the ability of NF-B to bind to the proximal CX₃CL1 promoter. Chromatin immunoprecipitation assays showed a 5-fold increase in the recruitment of NF-B to the CX₃CL1 gene promoter in response to IFN/TNF-; this too was reversed by dexamethasone. In contrast, dexamethasone did not displace NF-B from the granulocyte-macrophage colony-stimulating factor gene promoter. We conclude that CX₃CL1 expression is regulated through the NF-B pathway and that dexamethasone inhibits CX₃CL1 expression through a glucocorticoid receptor-dependent (RU486 sensitive) mechanism. This study also provides support for the action of glucocorticoids mediating their suppressive effects on expression by interfering with the binding of transcriptional activators at native gene promoters.—Bhavsar, P. K., Sukkar, M. B., Khorasani, N., Lee, K.-Y., Chung, K. F. Glucocorticoid suppression of CX₃CL1 (fractalkine) by reduced gene promoter recruitment of NF-B.

Key Words: epithelial cells • transcription factor • displacement • chromatin