HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: fj.07-9998comv1 fj.07-9998comv2 22/5/1606    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Dou, G.-R. Articles by Han, H. PubMed PubMed Citation Articles by Dou, G.-R. Articles by Han, H.

RBP-J, the transcription factor downstream of Notch receptors, is essential for the maintenance of vascular homeostasis in adult mice

Guo-Rui Dou^*,,1, Yao-Chun Wang^*,1, Xing-Bin Hu^*,1, Li-Hong Hou^*, Chun-Mei Wang^*, Jian-Feng Xu, Yu-Sheng Wang^,1,2, Ying-Min Liang^*,, Li-Bo Yao^*, An-Gang Yang^* and Hua Han^*,,2

^* State Key Laboratory of Cancer Biology, Department of Medical Genetics and Developmental Biology;

Department of Ophthalmology, Xijing Hospital; and

Department of Hematology, Tangdu Hospital, Fourth Military Medical University, Xi’an, China

²Correspondence: H.H., Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, Chang-Le Xi St. #17, Xi’an 710032, China. E-mail: huahan{at}fmmu.edu.cn; Y.S.W., Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China. E-mail: wangys{at}fmmu.edu.cn.com

In adults, angiogenic abnormalities are involved in not only tumor growth but several human inherited diseases as well. It is unclear, however, concerning how the normal vascular structure is maintained and how angiogenesis is initiated in normal adults. Using the Cre-LoxP-mediated conditional gene deletion, we show in the present study that in adult mice disruption of the transcription factor recombination signal-binding protein J (RBP-J) in endothelial cells strikingly induced spontaneous angiogenesis in multiple tissues, including retina and cornea, as well as in internal organs, such as liver and lung. In a choroidal neovascularization model, which mimics the angiogenic process in tumor growth and age-related macular degeneration, RBP-J deficiency induced a more intensive angiogenic response to injury. This could be transmitted by bone marrow, indicating that RBP-J could modulate bone marrow-derived endothelial progenitor cells in adult angiogenesis. In addition, in the absence of RBP-J, proliferation of endothelial cells increased significantly, leading to accumulative vessel outgrowth. These findings suggest that in adults RBP-J-mediated Notch signaling may play an essential role in the maintenance of vascular homeostasis by repressing endothelial cell proliferation.—Dou, G.-R., Wang, Y.-C., Hu, X.-B., Hou, L.-H., Wang, C.-M., Xu, J.-F., Wang, Y.-S., Liang, Y.-M., Yao, L.-B., Yang, A.-G., Han, H. RBP-J, the transcription factor downstream of Notch receptors, is essential for the maintenance of vascular homeostasis in adult mice.

Key Words: angiogenesis • Notch signaling • endothelial cells • choroidal neovascularization