HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: fj.07-9034comv1 22/2/522    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fainaru, O. Articles by Folkman, J. Search for Related Content PubMed PubMed Citation Articles by Fainaru, O. Articles by Folkman, J.

Dendritic cells support angiogenesis and promote lesion growth in a murine model of endometriosis

Ofer Fainaru^*,,1, Avner Adini^*, Ofra Benny^*, Irit Adini^*, Sarah Short^*, Lauren Bazinet^*, Kei Nakai^*, Elke Pravda^*, Mark D. Hornstein, Robert J. D'Amato^* and Judah Folkman^*

^* Department of Surgery, Vascular Biology Program, Children’s Hospital, and

Department of Obstetrics and Gynecology, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA

¹ Correspondence: Vascular Biology Program, Children’s Hospital Boston, Harvard Medical School, 300 Longwood Ave., Boston, MA 02115, USA. E-mail: ofer.fainaru{at}childrens.harvard.edu

Endometriosis affects 10–15% of women and is associated with pelvic pain and infertility. Angiogenesis plays an essential role in its pathogenesis. Dendritic cells (DCs) were recently implicated in supporting tumor angiogenesis. As both tumors and endometriosis lesions depend on angiogenesis, we investigated the possibility that DCs may also play a role in endometriosis. We induced endometriosis in 8-wk-old female C57BL/6 mice by implantation of autologous endometrium into the peritoneal cavity. We observed an abundance of CD11c⁺ DCs infiltrating sites of angiogenesis in endometriosis lesions. We noticed a similar pattern of infiltrating DCs at sites of angiogenesis in the peritoneal Lewis lung carcinoma tumor model. These DCs were immature (major histocompatability complex class II^low) and expressed vascular endothelial growth factor receptor 2. Peritoneal implanted bone marrow-derived DCs (BMDCs) incorporated into both endometriosis lesions and into B16 melanoma tumors and enhanced their growth at 8 days compared with controls (5.1±2.5 vs. 1.5±0.5 mm², n=4 and 4, P<0.0001 for endometriosis; 67.6±15.1 vs. 22.7±14.6 mm², n=5 and 7, P=0.0004 for mouse melanoma). Finally, immature BMDCs but not mature BMDCs enhanced microvascular endothelial cell migration in vitro (219±51 vs. 93±32 cells, P=0.02). Based on these findings, we suggest a novel role for DCs in supporting angiogenesis and promoting lesion growth both in endometriosis and in tumors.—Fainaru, O., Adini, A., Benny, O., Adini, I., Short, S., Bazinet, L., Nakai, K., Pravda, E., Hornstein, M. D., D’Amato, R. J., Folkman, J. Dendritic cells support angiogenesis and promote lesion growth in a murine model of endometriosis.

Key Words: Lewis lung carcinoma • melanoma

This article has been cited by other articles:

				K. Nakai, O. Fainaru, L. Bazinet, P. Pakneshan, O. Benny, E. Pravda, J. Folkman, and R. J. D'Amato Dendritic Cells Augment Choroidal Neovascularization Invest. Ophthalmol. Vis. Sci., August 1, 2008; 49(8): 3666 - 3670. [Abstract] [Full Text] [PDF]

				A. Van Langendonckt, J. Donnez, S. Defrere, G. A.J. Dunselman, and P. G. Groothuis Antiangiogenic and vascular-disrupting agents in endometriosis: pitfalls and promises Mol. Hum. Reprod., May 1, 2008; 14(5): 259 - 268. [Abstract] [Full Text] [PDF]