HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: fj.06-6884comv1 22/2/428    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Tanaka, K. Articles by Nagai, R. PubMed PubMed Citation Articles by Tanaka, K. Articles by Nagai, R.

Circulating progenitor cells contribute to neointimal formation in nonirradiated chimeric mice

Kimie Tanaka^*,||, Masataka Sata^*,,1, Takeshi Natori, Joo-ri Kim-Kaneyama, Kiyoshi Nose, Motoko Shibanuma, Yasunobu Hirata^* and Ryozo Nagai^*

^* Department of Cardiovascular Medicine,

Department of Advanced Clinical Science and Therapeutics, and

Department of Surgery, University of Tokyo Graduate School of Medicine, Tokyo, Japan;

Department of Microbiology, Showa University School of Pharmaceutical Sciences, Tokyo, Japan; and

^|| Japan Health Sciences Foundation, Tokyo, Japan

¹ Correspondence: Department of Cardiovascular Medicine, University of Tokyo Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan. E-mail: msata-circ{at}umin.net

Recent evidence suggests that bone marrow-derived cells may contribute to repair and lesion formation following vascular injury. In most studies, bone marrow-derived cells were tracked by transplanting exogenous cells into bone marrow that had been compromised by irradiation. It remains to be determined whether endogenous circulating progenitors actually contribute to arterial remodeling under physiological conditions. Here, we established a parabiotic model in which two mice were conjoined subcutaneously without any vascular anastomosis. When wild-type mice were joined with transgenic mice that expressed green fluorescent protein (GFP) in all tissues, GFP-positive cells were detected not only in the peripheral blood but also in the bone marrow of the wild-type mice. The femoral arteries of the wild-type mice were mechanically injured by insertion of a large wire. At 4 wk, there was neointima hyperplasia that mainly consisted of -smooth muscle actin-positive cells. GFP-positive cells were readily detected in the neointima (14.8±4.5%) and media (31.1±8.8%) of the injured artery. Some GFP-positive cells expressed -smooth muscle actin or an endothelial cell marker. These results indicate that circulating progenitors contribute to re-endothelialization and neointimal formation after mechanical vascular injury even in nonirradiated mice.—Tanaka, K., Sata, M., Natori, T., Kim-Kaneyama, J., Nose, K., Shibanuma, M., Hirata, Y., and Nagai, R. Circulating progenitor cells contribute to neointimal formation in nonirradiated chimeric mice.

Key Words: smooth muscle cell • irradiation • stem cells • vascular repair • parabiosis