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Dictyostelium discoideum—a promising expression system for the production of eukaryotic proteins

Ranjana Arya^*,1,2, Alok Bhattacharya and Kulvinder Singh Saini^*

^* Department of Biotechnology and Bioinformatics, Ranbaxy Laboratories Limited, Gurgaon, Haryana, India; and

School of Life Sciences, Jawaharlal Nehru University, New Delhi, India

² Correspondence: Department of Biotechnology and Bioinformatics, Ranbaxy Laboratories Limited, R&D III, Sector-18, Udyog Vihar, Gurgaon-122015, Haryana, India. E-mail: ranjanaa{at}mail.jnu.ac.in

In general, four different expression systems, namely, bacterial, yeast, baculovirus, and mammalian, are widely used for the overproduction of biochemical enzymes and therapeutic proteins. Clearly, bacterial expression systems offer ease of maneuverability with respect to large-scale production of recombinant proteins, while, a baculovirus expression system ensures proper protein modifications, processing, and refolding of complex proteins. Despite these advantages, mammalian cells remain the preferred host for many eukaryotic proteins of pharmaceutical importance, particularly, those requiring post-translational modifications. Recently, the single-celled slime mold, Dictyostelium discoideum (Dd), has emerged as a promising eukaryotic host for the expression of a variety of heterologous recombinant eukaryotic proteins. This organism possesses the complex cellular machinery required for orchestrating post-translational modifications similar to the one observed in higher eukaryotes. This review summarizes the advantages and disadvantages of Dictyostelium as an alternate system compared to other well-established expression systems. The key lessons learned from the expression of human recombinant proteins in this system are reviewed. Also, the strengths, weaknesses, and challenges associated with industrial-scale production of proteins in Dd expression system are discussed.—Arya, R., Bhattacharya, A., Saini, K. S. Dictyostelium discoideum—a promising expression system for the production of eukaryotic proteins.

Key Words: high-throughput system • recombinant • therapeutic • yield • phosphodiesterase 4D3