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Binding of elastin peptides to S-Gal protects the heart against ischemia/reperfusion injury by triggering the RISK pathway

Arnaud Robinet^*, Hervé Millart^*, Floriane Oszust^*, William Hornebeck^,1 and Georges Bellon

^* Laboratoire de Pharmacologie Médicale, Université de Reims-Champagne-Ardenne, Faculté de Médecine, Reims, France; and

Laboratoire de Biochimie et Biologie Moléculaire, Université de Reims-Champagne-Ardenne, Faculté de Médecine, UMR 6198, Centre National de la Recherche Scientifique, Reims, France

¹Correspondence: Laboratoire de Biochimie, Université de Reims-Champagne-Ardenne, Faculté de Médecine, CNRSUMR 6–19, 51, Rue Cognacq Jay, 51095 Reims cedex, France. E-mail: william.hornebeck{at}univ-reims.fr

Elastin peptides (EPs) generated by hydrolysis of elastic fibers by elastinolytic enzymes display a wide spectrum of biological activities. Here, we investigated their influence on rat heart ischemia-mediated injury using the Langendorff ex vivo model. EPs, i.e., kappa elastin, at 1.32- and 660-nM concentrations, when administered before the ischemia period, elicited a beneficial influence against ischemia by accelerating the recovery rate of heart contractile parameters and by decreasing significantly creatine kinase release and heart necrosis area when measured at the onset of the reperfusion. All effects were S-Gal-dependent, as being reproduced by (VGVAPG)₃ and as being inhibited by receptor antagonists, such as lactose and V14 peptide (VVGSPSAQDEASPL). EPs interaction with S-Gal triggered NO release and activation of PI₃-kinase/Akt and ERK1/2 in human coronary endothelial cells (HCAECs) and rat neonatal cardiomyocytes (RCs). This signaling pathway, as designated as RISK, for reperfusion injury salvage kinase pathway, was shown to be responsible for the beneficial influence of EPs on ischemia/reperfusion injury on the basis of its inhibition by specific pharmacological inhibitors. EPs survival activity was attained at a concentration averaging that present into the blood circulation, supporting the contention that these matrikines might offer a natural protection against cardiac injury in young and adult individuals. Such protective effect might be lost with aging, since we found that hearts from 24-month-old rats did not respond to EPs.—Robinet, A., Millart, H., Oszust, F., Hornebeck, W., Bellon, G. Binding of elastin peptides to S-Gal protects the heart against ischemia/reperfusion injury by triggering the RISK pathway.

Key Words: preconditioning • postconditioning • cardioprotection • aging