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Sulfide, the first inorganic substrate for human cells

Marc Goubern^*,1, Mireille Andriamihaja^*, Tobias Nübel, François Blachier^*,2 and Frédéric Bouillaud^,3

^* Ecole Pratique des Hautes Etudes and Nurélice UR909, INRA, F-78352 Jouy en Josas, France; and

UPR9078, CNRS Université René Descartes, F-75730 Paris, France

³Correspondence: CNRS-UPR9078, Université René Descartes, Site Necker 156 rue de Vaugirard, 75730 Paris Cedex 15, France. E-mail: bouillaud{at}necker.fr

Hydrogen sulfide (H₂S) is produced inside the intestine and is known as a poison that inhibits cellular respiration at the level of cytochrome oxidase. However, sulfide is used as an energetic substrate by many photo- and chemoautotrophic bacteria and by animals such as the lugworm Arenicola marina. The concentrations of sulfide present in their habitats are comparable with those present in the human colon. Using permeabilized colonic cells to which sulfide was added by an infusion pump we show that the maximal respiratory rate of colonocyte mitochondria in presence of sulfide compares with that obtained with succinate or L-alpha-glycerophosphate. This oxidation is accompanied by mitochondrial energization. In contrast, other cell types not naturally exposed to high concentration of sulfide showed much lower oxidation rates. Mitochondria showed a very high affinity for sulfide that permits its use as an energetic substrate at low micromolar concentrations, hence, below the toxic level. However, if the supply of sulfide exceeds the oxidation rate, poisoning renders mitochondria inefficient and our data suggest that an anaerobic mechanism involving partial reversion of Krebs cycle already known in invertebrates takes place. In conclusion, this work provides additional and compelling evidence that sulfide is not only a toxic compound. According to our study, sulfide appears to be the first inorganic substrate for mammalian cells characterized thus far.—Goubern, M., Andriamihaja, M., Nübel, T., Blachier, F., Bouillaud, F. Sulfide, the first inorganic substrate for human cells.

Key Words: mitochondria • oxidation • cytochrome oxidase • gut epithelium • colon

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