HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: fj.05-4144comv1 21/2/438    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wong, C.-H. Articles by Cheng, C. Y. Search for Related Content PubMed PubMed Citation Articles by Wong, C.-H. Articles by Cheng, C. Y.

Targeted and reversible disruption of the blood-testis barrier by an FSH mutant-occludin peptide conjugate

Ching-Hang Wong^*,1, Dolores D. Mruk^*,1, Will M. Lee and C. Yan Cheng^*,2

^* Center for Biomedical Research, The Population Council, New York, New York, USA; and

Department of Zoology, University of Hong Kong, Hong Kong, China

² Correspondence: Population Council, 1230 York Ave., New York, NY 10021, USA. E-mail: y-cheng{at}popcbr.rockefeller.edu

The blood-testis barrier (BTB) is one of the tightest blood-tissue barriers in mammals. As such, it poses a challenge to deliver any drugs to the seminiferous epithelium of the testis, such as a nonhormonal male contraceptive. To circumvent this problem, a genetically engineered follicle-stimulating hormone (FSH) mutant protein was produced in Spodoptera furgiperda (Sf)-9 insect cells to serve as a testis-specific carrier. Subsequently, a 22-amino acid peptide corresponding to the second extracellular loop of occludin, which was known to disrupt BTB integrity in vivo, was inserted to the FSH mutant by polymerase chain reaction (PCR), as well as chemical cross-linking. This molecule was found to have negligible hormonal activity but was still capable of binding to FSH receptors, which are restricted to Sertoli cells in mammals. When this FSH mutant-occludin peptide conjugate was administered to adult rats at 40 µg/adult rat (300 gm b.w.) via intraperitoneally (i.p.) injection, it induced transient and reversible disruption of the BTB, while at 150 µg/rat, it induced partial germ cell loss from the testis, particularly elongating/elongate spermatids. Most importantly, this effect was limited to the BTB without compromising the TJ-barrier integrity or cell adhesion in epithelia of other organs, such as kidney, liver, and small intestine. In summary, the use of an FSH mutant-occludin peptide conjugate is a feasible nanodevice to transiently compromise the BTB.—Wong, C.-H., Mruk, D. D., Lee, W. M., Cheng, C. Y. Targeted and reversible disruption of the blood-testis barrier by an FSH mutant-occludin peptide conjugate.

Key Words: spermatogenesis • tight junction • adherens junction • ectoplasmic specialization • serfoli-germ cell interactions • male contraception

This article has been cited by other articles:

				R. J. Mrsny, G. T. Brown, K. Gerner-Smidt, A. G. Buret, J. B. Meddings, C. Quan, M. Koval, and A. Nusrat A Key Claudin Extracellular Loop Domain is Critical for Epithelial Barrier Integrity Am. J. Pathol., April 1, 2008; 172(4): 905 - 915. [Abstract] [Full Text] [PDF]

				O. Sarkar, P. P. Mathur, C. Y. Cheng, and D. D. Mruk Interleukin 1 Alpha (IL1A) Is a Novel Regulator of the Blood-Testis Barrier in the Rat Biol Reprod, March 1, 2008; 78(3): 445 - 454. [Abstract] [Full Text] [PDF]