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Anti-inflammatory effect of interleukin-10 on human neutrophil respiratory burst involves inhibition of GM-CSF-induced p47^PHOX phosphorylation through a decrease in ERK1/2 activity

INSERM, U773, Centre Hospitalier Universitaire Xavier Bichat; Université Paris 7 Denis Diderot, Site Bichat; and AP-HP, CIB Phenogen, Paris, France

¹Correspondence: INSERM U773, Centre Hospitalier Universitaire Xavier Bichat, Faculté de Médecine, 16 rue Henri Huchard, Paris 75018, France. E-mail: dang{at}bichat.inserm.fr

ABSTRACT

Interleukin-10 (IL-10) exerts its anti-inflammatory properties by down-regulating polymorphonuclear neutrophil (PMN) functions such as reactive oxygen species (ROS) production via NADPH oxidase. The molecular mechanisms underlying this process are unclear. Partial phosphorylation of the NADPH oxidase cytosolic component p47^PHOX induced by proinflammatory cytokines, such as granulocyte-macrophage colony stimulating factor (GM-CSF) and tumor necrosis factor (TNF)-, is essential for priming ROS production by PMN. The aim of this study was to determine whether IL-10 inhibits GM-CSF- and TNF-induced p47^PHOX phosphorylation and to investigate the molecular mechanisms involved in this effect. We found that IL-10 selectively inhibited GM-CSF- but not TNF-induced p47^PHOX phosphorylation in a concentration-dependent manner. As GM-CSF-induced p47^PHOX phosphorylation is mediated by extracellular signal-regulated kinase 1/2 (ERK1/2), we tested the effect of IL-10 on this pathway. We found that IL-10 inhibited GM-CSF-induced ERK1/2 activity in an immunocomplex kinase assay. This inhibitory effect was confirmed by analyzing the phosphorylation status of the endogenous substrate of ERK1/2, p90RSK, in intact PMN. Furthermore, IL-10 decreased ROS production by adherent GM-CSF-treated PMN in keeping with the higher ROS production observed in whole blood from IL-10 knockout mice compared to their wild-type counterparts. Together, these results suggest that IL-10 inhibits GM-CSF-induced priming of ROS production by inhibiting p47^PHOX phosphorylation through a decrease in ERK1/2 activity. This IL-10 effect could contribute to the tight regulation of NADPH oxidase activity at the inflammatory site.—Dang, P.M., Elbim, C, Marie, J.-C., Chiandotto, M., Gougerot-Pocidalo, M.-A., El-Benna, J. Anti-inflammatory effect of interleukin-10 on human neutrophil respiratory burst involves inhibition of GM-CSF-induced p47^PHOX phosphorylation through a decrease in ERK1/2 activity.

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