HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Summary Full Text Full Text (PDF) All Versions of this Article: fj.05-3882fjev1 20/8/1230    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schlueter, P. J. Articles by Duan, C. Search for Related Content PubMed PubMed Citation Articles by Schlueter, P. J. Articles by Duan, C.

Gene duplication and functional divergence of the zebrafish insulin-like growth factor 1 receptors

Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109; and Howard Hughes Medical Institute, University of Chicago, Chicago, IL 60637

¹Correspondence: Department of MCDB, University of Michigan, Kraus Natural Science Bldg., Ann Arbor, MI 48109, USA. E-mail: cduan{at}umich.edu

ABSTRACT

Insulin-like growth factor (IGF) 1 receptor (IGF1R)-mediated signaling plays key roles in growth, development, and physiology. Recent studies have shown that there are two distinct ig f1r genes in zebrafish, termed ig f1ra and ig f1rb. In this study, we tested the hypothesis that zebrafish ig f1ra and ig f1rb resulted from a gene duplication event at the ig f1r locus and that this has led to their functional divergence. The genomic structures of zebrafish ig f1ra and ig f1rb were determined and their loci mapped. While zebrafish ig f1ra has 21 exons and is located on linkage group (LG) 18, zebrafish ig f1rb has 22 exons and mapped to LG 7. There is a strong syntenic relationship between the two zebrafish genes and the human IG F1R gene. Using a MO-based loss-of-function approach, we show that both Igf1ra and Igf1rb are required for zebrafish embryo viability and proper growth and development. Although Igf1ra and Igf1rb demonstrated a large degree of functional overlap with regard to cell differentiation in the developing eye, inner ear, heart, and muscle, they also exhibited functional distinction involving a greater requirement for Igf1rb in spontaneous muscle contractility. These findings suggest that the duplicated zebrafish ig f1r genes play largely overlapping but not identical functional roles in early development and provide novel insight into the functional evolution of the IGF1R/insulin receptor gene family.—Schlueter, P. J., Royer, T., Mohamed, H. F., Laser, B., Chan, S. J., Steiner, D. F., Duan, C. Gene duplication and functional divergence of the zebrafish insulin-like growth factor 1 receptors.

This article has been cited by other articles:

				B. E. Shmukler, J. S. Clark, A. Hsu, D. H. Vandorpe, A. K. Stewart, C. E. Kurschat, S.-K. Choe, Y. Zhou, J. Amigo, B. H. Paw, et al. Zebrafish ae2.2 encodes a second slc4a2 anion exchanger Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R1081 - R1091. [Abstract] [Full Text] [PDF]