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Systemic NO production during (septic) shock depends on parenchymal and not on hematopoietic cells: in vivo iNOS expression pattern in (septic) shock

Jennyfer Bultinck^*, Patrick Sips^*, Luc Vakaet, Peter Brouckaert^* and Anje Cauwels^*,1

^* Department for Molecular Biomedical Research, Ghent University and VIB, Ghent, Belgium; and

Department of Radiotherapy, Ghent University Hospital, Ghent, Belgium

¹Correspondence: Department for Molecular Biomedical Research, Ghent University/VIB, Technologiepark 927, Ghent 9052, Belgium. E-mail: anje.cauwels{at}dmbr.ugent.be

ABSTRACT

Septic shock is the leading cause of death in noncoronary intensive care units and the 10^th leading cause of death overall. Several lines of evidence support an important role for the vasodilator NO in hypotension, a hallmark of septic shock. However, NO may also positively or negatively regulate inflammation, apoptosis, and oxidative stress. These dual effects of NO may relate to its isoform specific production but also to differences in cellular and/or temporal expression. Via bone marrow transplantations, we examined the contribution of hematopoietic cells to the dramatically elevated NO levels seen in (septic) shock. Surprisingly, hematopoietic cells are not responsible at all for the production of circulating NO after systemic tumor necrosis factor or lipopolysaccharide challenge and contribute only marginally in a bacteremic (Salmonella) model of septic shock. Immunohistochemistry identified the nonhematopoietic sources of NO as hepatocytes, paneth cells, and intestinal and renal epithelial cells. In contrast, during granulomatous Bacillus Calmette-Guérin inflammation, the hematopoietic cell population represents the sole source of systemic NO. These mouse data demonstrate that, in contrast to the general conjecture, the dramatically elevated levels of NO during (septic) shock are not produced by hematopoietic cells such as monocytes/macrophages but rather by parenchymal cells in liver, kidney and gut.—Bultinck, J., Sips, P., Vakaet, L., Brouckaert, P., Cauwels, A. Systemic NO production during (septic) shock depends on parenchymal and not on hematopoietic cells: in vivo iNOS expression pattern in (septic) shock

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